不同制备方式对小剂量去白细胞悬浮红细胞标示量的影响分析  

Influence of different packaging methods on the volume of low-dose suspended leucocyte depleted RBCs

作　　者：陈少彬[1] 何子毅[1] 张春莲 陈庆恺[1] 张旭芬 李姗丹[1] 黄衬南[1] CHEN Shaobin;HE Ziyi;ZHANG Chunlian;CHEN Qingkai;ZHANG Xufen;LI Shandan;HUANG Chennan(Dongguan Central Blood Station,Dongguan 523000,China;Department of Neonatology,DongGuan Tungwah Hospital)

机构地区：[1]东莞市中心血站,广东东莞523000 [2]东莞东华医院新生儿科

出　　处：《中国输血杂志》2022年第5期562-565,共4页Chinese Journal of Blood Transfusion

基　　金：东莞市社会科技发展(重点)项目(201950715026187)。

摘　　要：目的 探讨不同制备方法对0.5 U小剂量去白细胞悬浮红细胞容量的影响,为精确标示小剂量去白细胞悬浮红细胞容量提供参考依据。方法 随机抽取母袋为1.5 U和2 U的去白悬浮红细胞,测定各袋重量和血液比重,计算血液容量,推断不同母袋分装0.5 U血液的重量与容量的关系及线性回归方程,用该回归方程计算和分析实际工作中3种不同制备方法(A手工多袋平均分装、B仪器多袋平均分装、C手工单袋分装)制备0.5 U去白悬浮红细胞的容量差异。结果 抽取38袋1.5 U和39袋2 U去白悬浮红细胞测定的比重分别为(1.090±0.011)g/mL和(1.097±0.013)g/mL,差异有统计学意义(P<0.05),从不同母袋(1.5 U和2 U)平均分装成0.5 U后,0.5 U的容量(Y)与整袋毛重(X)的回归方程分别为:Y_(1.5U分装)=0.902 7X-12.52(P<0.05)和Y_(2U分装)=0.905 6X-13.15(P<0.05);在实际血液制备中,母袋1.5 U采用方法A和B分装的血液容量均值(62.12±5.38)mL和(62.50±6.77)mL分别小于来自母袋2 U用方法A、B平均分装的容量(67.72±3.81)mL和(68.39±6.44)mL(P均<0.05),母袋不同时小剂量血液的差值范围:5.593~5.887 mL,且方法B的容量偏差(10.84%和9.42%)大于方法A(8.67%和5.63%);采用方法C从母袋1.5 U和2 U单袋分装的容量均值分别为(65.49±1.72)mL和(64.99±1.91)mL,差异无统计学意义(P>0.05),容量偏差分别为2.63%和2.94%;汇总全部0.5 U(n=483)的容量均值为(65.35±5.34)mL。结论 仪器平均分装法制备0.5 U的容量偏差最大,手工平均分装法次之,单袋分装法的容量差异最小;小剂量去白悬浮红细胞的容量标示量应根据不同母袋的规格及血液比重制定不同的制备方式。Objective To investigate the influence of different packaging methods on the volume of low-dose(0.5 U) suspended leucocyte depleted red blood cells(SLD RBC) and provide reference for accurate labeling.Methods Bags of SLD RBC in 1.5 U and 2 U were randomly sampled to measure the weight and specific gravity of each bag, so as to estimate the blood volume.The relationship between the weight and volume of 0.5 U blood, split from different parent bags, was analyzed and the linear regression equation was put forward.The regression equation was used to calculate and analyze the difference in the volume of 0.5 U SLD RBC prepared by three different packaging methods(A:manual multi-bag average packing;B:instrument multi-bag average packing;C:manual single-bag packing) in actual work.Results The specific gravity of 1.5 U(38 bags) and 2 U SLD RBC(39 bags) were(1.090±0.011) g/mL and(1.097±0.013) g/mL,respectively, and the difference was statistically significant(P<0.05).After the 0.5 U subsidiary bags were split from the parent bags(1.5 U or 2 U),the regression equations for the volume(Y) of 0.5 U and gross weight(X) of the whole bag were respectively: Y_(1.5 U packing)=0.902 7X-12.52(P<0.05) and Y_(2 U packing)=0.905 6X-13.15(P<0.05).In actual blood packaging, the average blood volume of 0.5 U subsidiary bags split from 1.5 U bag, using method A and B,were smaller than those split from 2 U bag [(62.12±5.38) mL and(62.50±6.77) mL vs(67.72±3.81) mL and(68.39±6.44)mL](P<0.05),with the deviation of low-dose blood volume from 5.593 mL to 5.887 mL.The volume deviation by method B(10.84% and 9.42%) were greater than that by method A(8.67% and 5.63%).The average volume of 0.5 U subsidiary bags split from 1.5 U and 2 U by method C were(65.49±1.72) mL and(64.99±1.91) mL(P>0.05),with volume deviation at 2.63% and 2.94%,respectively.The mean volume value of overall 0.5 U blood(n=483) was(65.35±5.34) mL.Conclusion For packaging 0.5 U subsidiary bags, the instrument multi-bag packaging showed the largest volume deviation, followe

关 键 词：小剂量 去白悬浮红细胞 容量 血液制备 分装方法 

分 类 号：R331.141[医药卫生—人体生理学] TQ645.56[医药卫生—基础医学]