桂枝茯苓丸对原发性痛经大鼠子宫组织NF-κB/COX-2/PGF_(2α)通路的影响  

Effect of Guizhi Fuling Pill on NF-κB/COX-2/PGF_(2α) pathway in uterine tissue of rats with primary dysmenorrhea

作　　者：刘灿纹 唐标[1] LIU Canwen;TANG Biao(Medical School,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China)

机构地区：[1]湖南中医药大学医学院,湖南长沙410208

出　　处：《湖南中医药大学学报》2022年第6期873-877,共5页Journal of Hunan University of Chinese Medicine

基　　金：湖南省中医药科研计划项目(2021215)。

摘　　要：目的观察桂枝茯苓丸对原发性痛经(primary dysmenorrhea,PD)大鼠子宫组织核因子κB(nuclear factor-κB,NF-κB)/环氧化酶-2(cyclooxygenase-2,COX-2)/前列腺素F_(2α)(prostaglandin F_(2α),PGF_(2α))通路的影响,探讨桂枝茯苓丸干预PD的机制。方法SD雌性大鼠随机分为正常组、模型组、桂枝茯苓丸组、布洛芬组,每组10只。采用苯甲酸雌二醇联合缩宫素建立PD大鼠模型。桂枝茯苓丸组、布洛芬组分别灌胃桂枝茯苓丸310 mg/kg、布洛芬1 mg/只;正常组、模型组均灌胃生理盐水10 mL/kg。灌胃10 d后,第11天记录各组大鼠扭体次数和潜伏期;观察子宫形态改变;ELISA法检测子宫组织中PGF_(2α)含量;Western blot检测子宫组织COX-2、NF-κB和磷酸化的NF-κB的水平。结果与正常组相比,模型组大鼠出现扭体反应,扭体次数显著增多(P<0.01),扭体潜伏期显著延长(P<0.01),子宫内膜大范围剥脱并有较严重的水肿,子宫组织PGF_(2α)含量以及COX-2、NF-κB以及磷酸化的NF-κB水平显著升高(P<0.01)。与模型组比较,桂枝茯苓丸组大鼠扭体次数显著减少(P<0.01),扭体潜伏期显著延长(P<0.01),子宫内膜剥脱少见且水肿程度较轻,子宫组织PGF_(2α)含量、COX-2和磷酸化的NF-κB水平显著降低(P<0.01)。结论桂枝茯苓丸能有效干预PD大鼠,其作用机制可能与抑制PD大鼠子宫组织NF-κB/COX-2/PGF;通路有关。Objective To observe the effect of Guzhi Fuling Pill on nuclear factor-κB(NF-κB)/cyclooxygenase-2(COX-2)/prostaglandin F_(2α)(PGF_(2α))pathway in uterine tissue of rats with primary dysmenorrhea(PD),so as to explore the mechanism of Guzhi Fuling Pill on PD.Methods SD female rats were randomly divided into normal group,model group,Guzhi Fuling Pill group,and ibuprofen group,with ten rats in each group.The PD rats model was established by estradiol benzoate and oxytocin.Guzhi Fuling Pill group and ibuprofen group were gavaged with Guizhi Fuling Pill 310 mg/kg,ibuprofen 1 mg/rat;normal group and model group were gavaged with normal saline 10 mL/kg.After 10 days of intragastric administration of Guizhi Fuiling Pill for 10 days,the number of writhing and latency of rats in each group were recorded on the 11th day;the changes of rat uterus were observed;the level of PGF;was detected by ELISA;the levels of COX-2,NF-κB and phosphorylated NF-κB were analyzed by Western blot.Results Compared with normal group,rats in model group showed writhing reaction,the number of writhing of rats was significantly increased(P<0.01),and the writhing latency was significantly prolonged(P<0.01),extensive endometrium peeling and severe edema,and the content of PGF_(2α),COX-2,NF-κB and phosphorylated NF-κB in uterine tissue of rats was significantly increased(P<0.01).Compared with model group,the number of writhing of rats in Guizhi Fuling Pill group was significantly reduced(P<0.01),and the writhing latency was significantly prolonged(P<0.01),endometrial exfoliation was less frequent and edema was less severe,and the content of PGF_(2α),COX-2 and phosphorylated NF-κB in uterine tissue was significantly decreased(P<0.01).Conclusion Guizhi Fuiling Pill has a significant effect on the intervention of PD rats,and mechanism of action may be related to the inhibition of NF-κB/COX-2/PGF_(2α) pathway in PD rats uterine tissue.

关 键 词：桂枝茯苓丸 原发性痛经 前列腺素F 核因子ΚB 环氧化酶-2 

分 类 号：R285.5[医药卫生—中药学]