止麻消痰活血汤对老年心肌梗死大鼠神经调节蛋白-1/受体酪氨酸蛋白激酶erbB3通路及心室重构的影响  

Effects of Zhima Xiaotan Huoxue Decoction on the neuregulin-1/receptor tyrosine protein kinase erbB3 pathway and ventricular remodeling in aged myocardial infarction rats

作　　者：王蕊 WANG Rui(Department of Medicine,Zaozhuang Hospital of Traditional Chinese Medicine,Zaozhuang,Shandong 277000,China)

机构地区：[1]枣庄市中医医院内科,山东枣庄277000

出　　处：《安徽医药》2022年第7期1315-1319,I0002,共6页Anhui Medical and Pharmaceutical Journal

摘　　要：目的 探讨止麻消痰活血汤对老年心肌梗死大鼠神经调节蛋白-1(NRG-1)/受体酪氨酸蛋白激酶erbB3(ErbB3)通路及心室重构的影响。方法 研究起止时间为2019年2—10月,大鼠来源于上海邦耀生物公司。制备老年心肌梗死模型大鼠,以随机数字表法分为模型组、止麻消痰活血汤低中高剂量组(10 mL/kg、20 mL/kg、30 mL/kg)、地尔硫‰组(2.61 mg/kg),每组12只,另取12只SD老年大鼠设为假手术组,分组处理后,测定各组大鼠左心室质量指数;苏木精-伊红(HE)、马松(Mosson)染色分别检测大鼠心肌病理形态变化及心室纤维化程度;酶联免疫吸附测定(ELISA)检测大鼠血清转化生长因子-β1(TGF-β1)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平;蛋白质印迹法检测大鼠心肌NRG-1/ErbB3通路相关蛋白的表达。结果 与假手术组比较,模型组大鼠心肌纤维扭曲、断裂,心肌细胞肥大、坏死,可见炎性细胞浸润等病理损伤,且胶原纤维显著增多,纤维化严重,血清TGF-β1、IL-6及TNF-α水平、左心室质量指数[(23.27±1.84)‰比(12.23±0.85)‰]显著升高(P<0.05),心肌NRG-1/ErbB3通路相关蛋白NRG-1[(0.30±0.04)比(2.16±0.37)]、p-ErbB3/ErbB3[(0.09±0.02)比(1.02±0.20)]显著降低(P<0.05);与模型组比较,止麻消痰活血汤低、中、高剂量组、地尔硫‰组大鼠病理损伤及纤维化症状得到改善,血清TGF-β1、IL-6及TNF-α水平、左心室质量指数[(19.92±1.47)‰、(16.36±1.06)‰、(12.89±0.87)‰、(12.84±0.86)‰比(23.27±1.84)‰]降低(P<0.05),心肌NRG-1/ErbB3通路相关蛋白NRG-1[(0.94±0.18)、(1.27±0.23)、(2.09±0.31)、(2.11±0.33)比(0.30±0.04)]、p-ErbB3/ErbB3[(0.27±0.04)、(0.51±0.08)、(0.92±0.16)、(0.94±0.18)比(0.09±0.02)]升高(P<0.05),且止麻消痰活血汤组间有剂量依赖性(P<0.05);与地尔硫‰组相比,止麻消痰活血汤高剂量组大鼠各指标差异无统计学意义(P>0.05)。结论 止麻消痰活血汤可激活NRG-1/ErbB3通路,减�Objective To investigate the effect of Zhima Xiaotan Huoxue decoction on the neuregulin-1/receptor tyrosine-protein kinase erbB3(NRG-1/ErbB3) pathway and ventricular remodeling in aged myocardial infarction rats.Methods ended from February to October 2019,and the rats were sourced from Shanghai Bangyao Biological Company.The aged myocardial infarction model rats were prepared and randomly divided into the model group,Zhima Xiaotan Huoxue decoction low(10 mL/kg),medium(20 mL/kg) and high(30 mL/kg) dose groups,and diltiazem(2.61 mg/kg) group,with 12 rats in each group,and another 12 SpragueDawley(SD) rats were selected as the sham operation group.The left ventricular mass index of the rats in each group was measured.Hematoxylin-eosin(HE) staining and Masson staining were used to detect the pathological changes in the myocardium and the degree of ventricular fibrosis.The levels of serum transforming growth factor-β1(TGF-β1),interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) were detected by enzyme-linked immunosorbent assay(ELISA).The expression of NRG-1/ErbB3 pathway-related proteins in the rat myocardium was detected by Western blotting.Results group were twisted and broken,the myocardial cells were hypertrophied and necrotic,inflammatory cell infiltration and other pathological damage were observed,collagen fibers were significantly increased,and fibrosis was severe.Serum levels of TGF-β1,IL-6 and TNF-α and the left ventricular mass index [(23.27±1.84)‰ vs.(12.23±0.85)‰] were significantly increased(P < 0.05).The myocardial NRG-1/ErbB3 pathway-related proteins NRG-1 [(0.30±0.04) vs.(2.16±0.37)] and p-ErbB3/ErbB3 [(0.09±0.02) vs.(1.02±0.20)] were significantly decreased(P < 0.05).Compared with the diltiazem group,the pathological damage and fibrosis symptoms of the Zhima Xiaotan Huoxue decoction low-,medium-and high-dose groups and the thiazolone group were improved,and the levels of serum TGF-β1,IL-6and TNF-α and the left ventricular mass index [(19.92±1.47)‰,(16.36±1.06)‰,(12.89±0.87

关 键 词：心肌梗死 止麻消痰活血汤 神经调节蛋白-1 受体酪氨酸蛋白激酶erbB3 心室重构 大鼠 Sprague-Dawley 

分 类 号：R285.5[医药卫生—中药学]