鱼藤素上调Kruppel样因子15表达影响卵巢癌SKOV细胞的增殖和凋亡  

Effect of deguelin on the proliferation and apoptosis of ovarian cancer SKOV cells by up-regulating the expression of KLF15

作　　者：赵莹 丁俊姗 杨慧丽[3] ZHAO Ying;DING Junshan;YANG Huili(Medical Examination Center,Henan Provincial Chest Hospital,Zhengzhou,Henan 450000,China;The Second District of Gynaecology,Zhengzhou Maternal and Child Health Hospital,Zhengzhou,Henan 450000,China;The Second District of Obstetrics,Zhengzhou Maternal and Child Health Hospital,Zhengzhou,Henan 450000,China)

机构地区：[1]河南省胸科医院体检中心,河南郑州450000 [2]郑州市妇幼保健院妇科二区,河南郑州450000 [3]郑州市妇幼保健院产科二区,河南郑州450000

出　　处：《安徽医药》2022年第7期1437-1441,共5页Anhui Medical and Pharmaceutical Journal

摘　　要：目的 探讨鱼藤素对卵巢癌SKOV细胞增殖和凋亡的影响及可能的作用机制。方法 本研究于2019年3月至2020年3月进行,鱼藤素作用SKOV细胞后,细胞计数试剂盒-8(CCK-8)检测细胞增殖,流式细胞仪检测细胞凋亡,蛋白免疫印迹(Western blotting)法分别对细胞中细胞周期蛋白依赖性激酶抑制剂1(P21)、细胞周期蛋白D1(CyclinD1)、B淋巴细胞瘤-2(Bcl-2)及Bcl-2相关X蛋白(Bax)、Kruppel样因子15(KLF15)蛋白表达水平进行检测,实时荧光定量PCR(RT-qPCR)检测KLF15mRNA表达水平。转染KLF15过表达载体上调SKOV细胞中KLF15表达后,上述相同方法观察上调KLF15表达对SKOV细胞增殖、凋亡及CyclinD1、P21、Bcl-2和Bax蛋白表达的影响。结果 鱼藤素作用SKOV3细胞后,细胞活性(0.49±0.04、0.39±0.03、0.30±0.02)、CyclinD1(0.53±0.05、0.45±0.05、0.21±0.03)及Bcl-2(0.40±0.04、0.24±0.02、0.16±0.01)蛋白表达水平降低(P<0.05),细胞凋亡率[(6.63±0.69)%、(12.71±1.06)%、(20.27±2.25)%]、P21(0.23±0.02、0.46±0.04、0.74±0.03)、Bax(0.20±0.02、0.36±0.02、0.55±0.05)蛋白表达水平升高,KLF15 mRNA和蛋白表达水平升高(P<0.05)。上调KLF15表达后,SKOV3细胞活性、CyclinD1及Bcl-2蛋白表达水平降低(P<0.05),细胞凋亡率、P21及Bax蛋白表达水平升高(P<0.05)。下调KLF15表达后,鱼藤素作用的SKOV3细胞活性、CyclinD1及Bcl-2蛋白表达水平升高(P<0.05),细胞凋亡率、P21及Bax蛋白表达水平降低(P<0.05)。结论鱼藤素可抑制卵巢癌SKOV3细胞增殖,并诱导SKOV3细胞凋亡,其作用机制与上调细胞中KLF15表达有关。Objective To investigate the effect of deguelin on the proliferation and apoptosis of ovarian cancer SKOV cells and its possible mechanism.Methods Experiments were conducted from March 2019 to March 2020.After deguelin acted on SKOV cells,Cell counting kit-8(CCK-8) was used to detect cell proliferation,flow cytometry was used to detect cell apoptosis,and Western blotting was used to detect cyclin-dependent kinase inhibitor 1(P21),Cyclin D1,B-lymphoma-2(Bcl-2),Bcl-2-related X protein(Bax),Kruppel-like factor 15(KLF15) protein expression levels were detected,real-time quantitative PCR(RT-qPCR)) to detect the expression level of KLF15 mRNA.After transfecting KLF15 overexpression vector to up-regulate KLF15 expression in SKOV cells,the same methods as above were used to observe the effect of up-regulating KLF15 expression on SKOV cell proliferation,apoptosis and the expression of CyclinD1,P21,Bcl-2 and Bax protein.Results After deguelin acted on SKOV3 cells,cell activity(0.49±0.04,0.39±0.03,0.30±0.02),protein expression levels of CyclinD1(0.53±0.05,0.45±0.05,0.21±0.03) and Bcl-2(0.40±0.04,0.24±0.02),0.16±0.01) were decreased(P<0.05),apoptosis rate [(6.63±0.69)%,(12.71±1.06)%,(20.27±2.25)% ],protein expression levels of P21(0.23±0.02,0.46±0.04,0.74±0.03),Bax(0.20±0.02,0.36±0.02,0.55±0.05) protein expression level increased,KLF15 mRNA and protein expression levels increased(P<0.05).After up-regulating KLF15 expression,SKOV3 cell activity,protein expression levels of CyclinD1 and Bcl-2 were decreased(P<0.05),apoptosis rate,protein expression levels of P21 and Bax were increased(P<0.05).After down-regulating the expression of KLF15,the SKOV3 cell activity,CyclinD1 and bcl-2 protein expression levels of cells increased by treating deguelin(P<0.05),the apoptosis rate,P21 and Bax protein expression levels were decreased(P<0.05).Conclusions Deguelin can inhibit the proliferation of ovarian cancer SKOV3 cells and induce the apoptosis of SKOV3 cells,and its mechanism is related to the up-regulation of KLF15 e

关 键 词：黄酮类 卵巢肿瘤 鱼藤素 Kruppel样因子15 细胞增殖 凋亡 

分 类 号：R285[医药卫生—中药学]