重离子辐照诱变增强枯草芽孢杆菌纤维素降解能力  

Breeding of a high-cellulose degrading Bacillus subtilis by heavy ion mutagenesis

作　　者：张丽敏 李永丽[1,2,3] 胡建华 崔金娜[1,2,3] 刘占英 朱明达 ZHANG Limin;LI Yongli;HU Jianhua;CUI Jinna;LIU Zhanying;ZHU Mingda(School of Chemical Engineering,Inner Mongolia University of Technology,Hohhot 010051,China;Center for Energy Conservation and Emission Reduction in Fermentation Industry in Inner Mongolia,Hohhot 010051,China;Engineering Research Center of Inner Mongolia for Green Manufacturing in Bio-fermentation Industry,Hohhot 010051,China)

机构地区：[1]内蒙古工业大学化工学院,呼和浩特010051 [2]内蒙古自治区发酵产业节能减排工程技术研究中心,呼和浩特010051 [3]生物发酵绿色制造内蒙古自治区工程研究中心,呼和浩特010051

出　　处：《辐射研究与辐射工艺学报》2022年第3期60-68,共9页Journal of Radiation Research and Radiation Processing

基　　金：科技厅科技重大专项(2019ZD021)资助。

摘　　要：为了提高枯草芽孢杆菌纤维素降解能力,本实验从5株枯草芽孢杆菌中筛选出一株纤维素降解率较高的菌株作为出发菌株,利用12C6+离子束辐照诱变处理后,采用刚果红染色法进行初筛,通过测定纤维素降解率及内切葡聚糖苷酶活力进行复筛,对选出的高纤维素降解率菌株进行发酵条件优化。最终得到一株Bacillus subtilis CG-40-1突变株,其纤维素降解率为(53.07±0.75)%,较出发菌株提高了17.75%,并具有良好的纤维素降解稳定性。经过发酵条件优化,最终得到该菌株的最适发酵时间、温度和pH分别为120 h、45℃、6.5,纤维素降解率为(67.79±0.49)%,较未优化时提高27.74%。To improve the cellulose degradation ability of Bacillus subtilis(B. subtilis), a strain with a higher cellulose degradation rate was selected from five B. subtilis strains as the starting strain in this experiment. By heavy ion irradiation mutagenesis treatment, we screened a high cellulose degradation rate strain, and optimized its fermentation conditions. The results showed that a B. subtilis CG-40-1 mutant strain cellulose degradation rate was(53.07±0.75)%, which was 17.75% higher than the starting strain and has good genetic stability. After the optimization experiment of fermentation conditions, the optimal fermentation time, culture temperature, and pH of this strain were 120 h, 45 ℃, and 6.5, respectively. And the cellulose degradation rate was(67.79±0.49)%, which was 27.74% higher than that without optimization.

关 键 词：枯草芽孢杆菌 重离子 辐照 诱变 纤维素降解率 发酵过程优化 

分 类 号：Q31[生物学—遗传学]