拟南芥突变体 myc234 的鉴定及其对MeJA的敏感性分析  

Identification and MeJA Sensitivity Analysis of Arabidopsis Mutant myc 234

作　　者：王炎炎[1] 张斐斐[2] 熊娟 王雪 李林 冯蕊 董萍萍 殷伊君 张有刚[4] 周丽娟 WANG Yanyan;ZHANG Feifei;XIONG Juan;WANG Xue;LI Lin;FENG Rui;DONG Pingping;YIN Yijun;ZHANG Yougang;ZHOU Lijuan(International Agricultural Research Institute,Yunnan Academy of Agricultural Sciences,Kunming,Yunnan,China 650205;Biotechnolgy and Germplasm Resources Institute,Yunnan Academy of Agricultural Sciences,Kunming,Yunnan,China 650205;School of Agriculture and Life Sciences,Kunming University,Kunming,Yunnan,China 650214;School of Energy and Environment Science,Yunnan Normal University,Kunming,Yunnan,China 650504)

机构地区：[1]云南省农业科学院国际农业研究所,云南昆明650205 [2]云南省农业科学院生物技术与种质资源研究所,云南昆明650205 [3]昆明学院农学与生命科学学院,云南昆明650214 [4]云南师范大学能源与环境科学学院,云南昆明650504

出　　处：《昆明学院学报》2022年第3期100-104,共5页Journal of Kunming University

基　　金：云南省农业基础研究联合专项资助项目(2017FG001(-085));云南省地方本科高校基础研究联合专项项目(2018FH001-025);昆明学院人才引进资助项目(YJL18005);云南省教育厅科学研究基金项目(2021Y722);云南省大学生创新项目(202011393010);云南省大学生创新项目(202111393007).

摘　　要：AtMYC 2(AT1G32640),AtMYC 3(AT5G46760)和AtMYC 4(AT4G17880)是茉莉酸信号通路的关键基因,为深入挖掘其在拟南芥生长发育及防御反应中的基因功能,结合PCR,SqRT-PCR半定量及测序技术对myc 2,myc 3和myc 4单突变体杂交获得的F3代植株进行鉴定,获得7株纯合三突变体.并以40μmol/L MeJA对纯合三突材料进行外源处理.结果显示,野生型根长较对照降低39.57%,而myc 234纯合三突变体较对照升高3.54%,说明其对MeJA敏感度明显下降.研究结果可为myc 234突变体的鉴定以及对茉莉酸敏感性相关研究提供试验依据及材料.AtMYC 2(AT1G32640),AtMYC 3(AT5G46760)and At MYC 4(AT4G17880)were the key regulators involved in the jasmine acid signaling pathway.To further explore the gene functions during development and defense response in Arabidopsis thaliana,combined PCR,SqRT-PCR semi-quantitative and sequencing technology,F3 generation progenies obtained through the mutual hybridization and self-breeding of myc 2,myc 3 and myc 4 mutants were identified to get 7 homozygous triple mutants of stable genetic.The response sensitivity of the triple mutants to exogenous MeJA treatment of 40μmol/L was analyzed,the result showed that the root length of wild-type reduced 39.57%,while the growth of myc 234 triple mutants increased 3.54%.showing that the sensitivity to MeJA decreased significantly.This study provided experimental basis and material for the identification of myc 234 mutant and the study of jasmonin sensitivity.

关 键 词：拟南芥 MYC转录因子 突变体鉴定 MeJA敏感性 茉莉酸 SqRT-PCR 

分 类 号：Q78[生物学—分子生物学] Q945.4