Homology-based repair induced by CRISPR-Cas nucleases in mammalian embryo genome editing  

作　　者：Xiya Zhang Tao Li Jianping Ou Junjiu Huang Puping Liang 

机构地区：[1]Center for Reproductive Medicine,the Third Affiliated Hospital of Sun Yat-sen University,Sun Yat-sen University,Guangzhou 510630,China [2]MOE Key Laboratory of Gene Function and Regulation,State Key Laboratory of Biocontrol,School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China [3]Key Laboratory of Reproductive Medicine of Guangdong Province,the First Affiliated Hospital and School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China

出　　处：《Protein & Cell》2022年第5期316-335,共20页蛋白质与细胞（英文版）

基　　金：the Key Technologies Research and Development Program(2017YFC1001901);the Guangdong Special Support Program(2019BT02Y276);Guangdong Basic and Applied Basic Research Foundation(2018A030313370 and 2021A1515010759);the National Natural Science Foundation of China(31971365);the Guangzhou Science and Technology Project(201803010020).

摘　　要：Recent advances in genome editing,especially CRISPR-Cas nucleases,have revolutionized both laboratory research and clinical therapeutics.CRISPR-Cas nucleases,together with the DNA damage repair pathway in cells,enable both genetic diversification by classical non-homologous end joining(c-NHEJ)and precise genome modification by homology-based repair(HBR).Genome editing in zygotes is a convenient way to edit the germline,paving the way for animal disease model generation,as well as human embryo genome editing therapy for some life-threatening and incurable diseases.HBR efficiency is highly dependent on the DNA donor that is utilized as a repair template.Here,we review recent progress in improving CRISPR-Cas nuclease-induced HBR in mammalian embryos by designing a suitable DNA donor.Moreover,we want to provide a guide for producing animal disease models and correcting genetic mutations through CRISPR-Cas nuclease-induced HBR in mammalian embryos.Finally,we discuss recent developments in precise genome-modification technology based on the CRISPR-Cas system.

关 键 词：homology-based repair(HBR) genome editing disease modeling EMBRYO precision medicine 

分 类 号：Q78[生物学—分子生物学]