The nucleocapsid protein of rice stripe virus in cell nuclei of vector insect regulates viral replication  

作　　者：Wan Zhao Junjie Zhu Hong Lu Jiaming Zhu Fei Jiang Wei Wang Lan Luo Le Kang Feng Cui 

机构地区：[1]State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China [2]CAS Center for Excellence in Biotic Interactions,University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Protein & Cell》2022年第5期360-378,共19页蛋白质与细胞（英文版）

基　　金：the State Key Research Development Program of China(No.2019YFC1200504);the National Natural Science Foundation of China(No.32090012);the National Key R&D Program of China(No.2017YFD0200400);the Collaborative Program between Chinese Academy of Sciences and CSIRO of Australia(No.152111KYSB20190062);the Youth Innovation Promotion Association,CAS(No.2019086).

摘　　要：Rice stripe virus(RSV)transmitted by the small brown planthopper causes severe rice yield losses in Asian countries.Although viral nuclear entry promotes viral replication in host cells,whether this phenomenon occurs in vector cells remains unknown.Therefore,in this study,we systematically evaluated the presence and roles of RSV in the nuclei of vector insect cells.We observed that the nucleocapsid protein(NP)and viral genomic RNAs were partially transported into vector cell nuclei by utilizing the importin a nuclear transport system.When blocking NP nuclear localization,cytoplasmic RSV accumulation significantly increased.In the vector cell nuclei,NP bound the transcription factor YY1 and affected its positive regulation to FAIM.Subsequently,decreased FAIM expression triggered an antiviral caspase-dependent apoptotic reaction.Our results reveal that viral nuclear entry induces completely different immune effects in vector and host cells,providing new insights into the balance between viral load and the immunity pressure in vector insects.

关 键 词：rice stripe virus nucleocapsid protein nuclear localization importinα YY1 

分 类 号：S435.111[农业科学—农业昆虫与害虫防治]