草地贪夜蛾在玉米-小麦寄主转换中的转录组分析  

Transcriptome analysis of Spodoptera furgiperda during corn-wheat host alternation

作　　者：王斯亮 邵越 闫成进[1] WANG Siliang;SHAO Yue;YAN Chengjin(College of Agriculture and Biotechnology, Wenzhou Vocational College of Science and Technology, Wenzhou 325000, Zhejiang, China;Plant Protection and Soil Fertilizer Management Station of Wenzhou, Wenzhou 325000, Zhejiang, China)

机构地区：[1]温州科技职业学院农业与生物技术学院,浙江温州325000 [2]温州市植物保护与土壤肥料管理站,浙江温州325000

出　　处：《浙江农业学报》2022年第6期1236-1247,共12页Acta Agriculturae Zhejiangensis

基　　金：温州市重大科技创新攻关项目(ZN2019002);浙江省教育厅一般科研项目(Y201942802);温州科技职业学院博士启动项目。

摘　　要：草地贪夜蛾(Spodoptera furgiperda)是重大入侵多食性害虫,在我国南方地区可以周年繁殖。过去的调查和研究表明了小麦有成为草地贪夜蛾越冬寄主的风险。为了探究草地贪夜蛾取食小麦的适应性的内在机制,本研究通过转录组测序技术,以取食玉米的草地贪夜蛾幼虫为对照组,分析比较草地贪夜蛾在转换取食小麦和人工饲料后的表达谱差异。共组装得到66407条Unigene,其中18868条获得功能注释。取食小麦和人工饲料的幼虫中,分别找到542条和6863条差异表达基因。对差异表达基因进行分析,发现大量表皮相关基因表达量在取食小麦的幼虫中显著上调,而在取食人工饲料的幼虫中下调。同时发现细胞色素P450基因在取食小麦和人工饲料的幼虫中均有下调表达。本研究为草地贪夜蛾为害小麦越冬的风险评估工作提供信息和参考。Spodoptera furgiperda is a major invading pest.In the south of China,S.furgiperda can survive and multiply through whole year.Previous investigations and research revealed the risk that wheat was overwintering host of S.furgiperda.To investigate the internal mechanism of feeding fitness of S.furgiperda to wheat,transcriptomes of S.furgiperda larva feeding on corn,wheat and artificial diet were sequenced and compared.Totally,66407 unigenes were obtained after assembly and 18868 unigenes were functionally annotated.Respective 542 and 6863 differentially expressed genes were found in wheat-feeding larva and artificial-diet-feeding larva,comparing with corn-feeding larva.Analysis of differentially expressed genes showed that lots of cuticle-related genes up regulated in wheat-feeding larva,while down regulated in artificial-diet-feeding larva.Cytochrome P450 genes were found down regulated in both wheat-feeding and artificial-diet-feeding larva.This study provides information and reference to risk assessment that S.furgiperda damage wheat through overwintering stage.

关 键 词：草地贪夜蛾 转录组 取食 小麦 人工饲料 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]