条芩与枯芩的指纹图谱建立、抗炎活性及谱效关系研究  

Establishment of fingerprints,anti-inflammatory activities and spectrum-effect relationship study of pithnodecayed and pith-decayed Scutellaria baicalensis

作　　者：贾传青 郭兰萍[3] 王晓 于宗渊[4] 陈龙 董红敬 JIA Chuanqing;GUO Lanping;WANG Xiao;YU Zongyuan;CHEN Long;DONG Hongjing(College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Shandong Analysis and Test Center,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250014,China;National Resource Center for Chinese Materia Medica,China Academy of Chinese Material Science/State Key Laboratory of Genuine Medicinal Materials Breeding Base,Beijing 100700,China;Shandong Academy of Traditional Chinese Medicine,Jinan 250014,China)

机构地区：[1]山东中医药大学药学院,济南250355 [2]齐鲁工业大学(山东省科学院)山东省分析测试中心,济南250014 [3]中国中医科学院中药资源中心/道地药材国家重点实验室培育基地,北京100700 [4]山东省中医药研究院,济南250014

出　　处：《中国药房》2022年第12期1430-1435,共6页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81803662);中央本级重大增减支项目(No.2060302);山东省重点研发计划(重大科技创新工程)项目(No.2021CXGC010508)。

摘　　要：目的建立条芩与枯芩的指纹图谱,考察两者的抗炎活性差异。方法采用高效液相色谱法建立5批条芩和5批枯芩的指纹图谱;采用SIMCA 14.1软件,以变量重要性投影(VIP)值>1为标准筛选影响条芩和枯芩质量的差异标志物;以其对脂多糖(LPS)诱导的小鼠单核巨噬细胞(RAW264.7细胞)上清液中一氧化氮(NO)、白细胞介素6(IL-6)、IL-1β的抑制率为指标,评价条芩和枯芩的抗炎活性并计算其抑制NO的半数有效浓度(EC50);采用灰色关联分析谱效关系;以50μg/mL质量浓度下各组细胞的NO、IL-1β、IL-6抑制率为指标,采用SPSS 26.0软件进行聚类分析。结果5批条芩和5批枯芩共有15个共有峰,相似度均大于0.990。共有8个峰的VIP值>1,依次为峰14、12、15、6、10、13、11、4。在50μg/mL质量浓度下,枯芩提取物对NO、IL-6、IL-1β的抑制率及平均EC50分别为62.14%~71.13%、3.32%~18.38%、93.12%~95.47%、25.35μg/mL,条芩提取物分别为39.52%~50.19%、6.21%~22.55%、94.10%~96.44%、58.63μg/mL;枯芩提取物的平均NO抑制率显著高于条芩提取物,平均EC50、IL-6抑制率均显著低于条芩提取物(P<0.05);两者平均IL-1β抑制率比较差异无统计学意义(P>0.05)。峰2~3、5~8、10~11与NO抑制率的关联度均大于0.8,峰2、5、8~9与IL-1β抑制率的关联度均大于0.9,15个共有峰与IL-6抑制率的关联度均小于0.8。10批样品可聚为2类,K1~K5为一类,T12~T16为一类。结论在50μg/mL质量浓度下,枯芩提取物对NO的抑制作用强于条芩提取物,对IL-6的抑制作用弱于条芩提取物,对IL-1β的抑制作用与条芩提取物相当;初步确定峰6、10~11对应成分为条芩与枯芩抗炎活性差异的主要化学成分。OBJECTIVE To establish the fingerprints of pith-nodecayed and pith-decayed Scutellaria baicalensis,and to investigate the difference of their anti-inflammatory activities. METHODS HPLC method was adopted to establish the fingerprints of 5 batches of pith-nodecayed S. baicalensis and 5 batches of pith-decayed S. baicalensis. SIMCA 14.1 software was adopted to screen the markers of weight difference between pith-decayed S. baicalensis and pith-nodecayed S. baicalensis,using variable importance in projection(VIP) >1 as standard. Using the inhibitory rates of NO,IL-6 and IL-1β in supernatant of lipopolysaccharide(LPS)-induced monocyte macrophages(RAW264.7 cells)as indexes,the anti-inflammatory activities of pith-decayed and pith-nodecayed S. baicalensis were evaluated and median effective concentration(EC50) of NO were calculated. The gray correlation method was used for spectrum-effect relationship analysis;SPSS 26.0 software was used for cluster analysis using the inhibitory rates of NO,IL-6 and IL-1β in cells at the concentration of 50 μg/mL. RESULTS There were 15 common peaks in 5 batches of pithnodecayed S. baicalensis and 5 batches of pithdecayed S. baicalensis,and the similarity of them was greater than 0.990. There were total of 8 peaks with VIP>1.0,they were peak 14, 12, 15, 6, 10, 13, 11 and 4. At the concentration of 50 μg/m L, the inhibitory rates of pithdecayed S. baicalensis extract to NO,IL-6,IL-1β and the average EC50 were 62.14%-71.13%,3.32%-18.38% and93.12%-95.47% and 25.35 μg/m L,respectively;those of pith-nodecayed S. baicalensis extract were 39.52%-50.19%,6.21%-22.55%,94.10%-96.44% and 58.63 μg/m L,respectively. Average NO inhibition rate of pith-decayed S. baicalensis extract was significantly higher than that of pith-nodecayed S. baicalensis extract;average EC50 and average IL-6 inhibitory rate of pithdecayed S. baicalensis extract were significantly lower than those of pith-nodecayed S. baicalensis extract(P<0.05). There was no statistical significance in average inhibitory rate of IL-1

关 键 词：黄芩 条芩提取物 枯芩提取物 指纹图谱 抗炎活性 谱效关系 

分 类 号：R285.5[医药卫生—中药学]