基于UPLC-QTOF-MS/MS的藏药翼首草不同提取物化学成分比较研究  

Comparative Study on Chemical Constituents in Different Extracts of Pterocephalus hookeri(C.B.Clarke)Hoeck by UPLC-QTOF-MS/MS Analysis

作　　者：黄美玲 周静[1,2] 伍城颖[1,2] 曾真[1,2] 李松林 钱士辉 Huang Meiling;Zhou Jing;Wu Chengying;Zeng Zhen;Li Songlin;Qian Shihui(Department of Pharmaceutical Analysis,Affiliated Hospital of Integrated Traditional Chinese and Western Medicine,Nanjing University of Traditional Chinese Medicine,Nanjing 210028,China;Department of Metabolomics,Jiangsu Province Academy of Traditional Chinese Medicine,Nanjing 210028,China;Jiangsu Province Platform for Conservation and Utilization of Agricultural Germplasm,Nanjing 210014,China)

机构地区：[1]南京中医药大学附属中西医结合医院,江苏南京210028 [2]江苏省中医药研究院中药质量与代谢组研究室,江苏南京210028 [3]江苏省农业种质资源保护与利用平台,江苏南京210014

出　　处：《中国野生植物资源》2022年第6期31-40,49,共11页Chinese Wild Plant Resources

基　　金：国家重点研发计划(2019YFC1711000);国家自然科学基金青年基金项目(82104529);江苏省中医药研究院青年科学研究项目(QNKXYJ202103)。

摘　　要：应用UPLC-QTOF-MS/MS鉴定翼首草化学成分,对其水提取物和醇提取物中差异性化合物进行辨识并进行半定量评价。从4批翼首草药材中共鉴定出48个化合物,其中5个化合物首次在翼首草发现。75%乙醇提取物及水提取物整体化学轮廓差异较大,从中辨识16个差异性化合物,其中4个(rivularicin等)化合物为醇提液中所特有。水提取物中neochlorogenic acid等4个化合物含量较高;75%乙醇提取物中sylvestroside III等8个化合物含量较高。该含量差异是否会对其药效产生影响仍有待进一步研究。Ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spec‑trometry(UPLC-QTOF-MS/MS)was used to identify the chemical components of Pterocephalus hookeri(C.B.Clarke)Hoeck(P.hookeri),distinguish the different compounds in its water extract(WE)and 75%ethanol extract(EE)of P.hookeri and conduct semi-quantitative evaluation.A total of 48 com‑pounds were identified from 4 batches of P.hookeri herbal medicine,5 compounds were found in P.hook⁃eri for the first time.The overall chemical profile of the 75%ethanol extract and the water extract is quite different,and 16 different compounds were identified,of which 4 compounds(rivularicin,etc)are only exist in EE.The content of 4 compounds including neochlorogenic acid in WE is higher;the content of 8 compounds including sylvestroside III in 75%EE is higher.Whether this content difference will affect its efficacy remains to be further studied.

关 键 词：翼首草 水提取物 醇提取物 UPLC-QTOF-MS/MS 差异成分 

分 类 号：R932[医药卫生—生药学]