腹腔镜激光散斑血流成像技术  

Laparoscopic Laser Speckle Blood Flow Imaging Technology

作　　者：吴琼 周伟[1] 徐宝腾 刘家林 杨西斌 王驰[2] 熊大曦 Wu Qiong;Zhou Wei;Xu Baoteng;Liu Jialin;Yang Xibin;Wang Chi;Xiong Daxi(Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences,Suzhou,Jiangsu 215163,China;School of Mechatronic Engineering and Automation,Shanghai University,Shanghai 200444,China;School of Biomedical Engineering,University of Science and Technology of China,Hefei,Anhui 230026,China)

机构地区：[1]中国科学院苏州生物医学工程技术研究所,江苏苏州215163 [2]上海大学机电工程与自动化学院,上海200444 [3]中国科学技术大学生物医学工程学院,安徽合肥230026

出　　处：《光学学报》2022年第7期236-243,共8页Acta Optica Sinica

基　　金：国家自然科学基金青年科学基金(61905272);国家重点研发计划(2018YFC0114800,2017YFC0109900);江苏省重点研发计划(BE2018666);中科院青年创新促进会项目(2018359);苏州市学科重点实验室项目(SZS2020308)。

摘　　要：激光散斑衬比成像(LSCI)技术广泛应用于大视场的组织表层血流成像,当需要实时在体监测生物体深层组织或腔内组织的血流分布及变化时,将LSCI与内镜成像技术结合是解决LSCI成像深度问题的有效途径。为此,搭建了商用腹腔镜LSCI成像系统,并对微流体仿体和兔子大肠进行成像。实验结果表明,所搭建的系统可以校正静态散射以及消除系统噪声对散斑衬比度的影响,利用单次曝光下的散斑衬比测量值可以实现血流的定量监测,该商用腹腔镜LSCI成像系统将具有重要的临床应用潜力。Laser speckle contrast imaging(LSCI)technology is widely used in tissue surface blood flow imaging with large field of view.When real-time in vivo monitoring of blood flow distribution and changes in deep tissue or lumen tissue is needed,the combination of LSCI and endoscopic imaging is an effective way to solve the problem of LSCI imaging depth.For this reason,a commercial laparoscopic LSCI imaging system is built to image microfluidic replicas and rabbit large intestine.The experimental results show that the system can correct the static scattering and eliminate the influence of system noise on speckle contrast,and the quantitative monitoring of blood flow can be realized by using the speckle contrast measurement under single exposure.The commercial laparoscopic LSCI imaging system will have important clinical application potential.

关 键 词：医用光学 散斑衬比 血流监测 内窥成像 静态散射 

分 类 号：O436[机械工程—光学工程]