离体小鼠股骨骨小梁与骨皮质CT样品制备探索  

Exploration on the Preparation of CT Samples of Isolated Mouse Femoral Trabecular Bone and Cortical Bone

作　　者：王战京 武文琦 赵媛媛 雷建锋 WANG Zhan-jing;WU Wen-qi;ZHAO Yuan-yuan;LEI Jian-feng(Core Facility Center,Capital Medical University,Beijing 100069,China)

机构地区：[1]首都医科大学中心实验室,北京100069

出　　处：《医学信息》2022年第12期118-121,共4页Journal of Medical Information

摘　　要：目的 探索不同的样品制备方法对离体小鼠股骨骨小梁密度(BMD)与骨皮质密度(TMD)CT定量测量的影响。方法 购入6~8周雌性BALA/C小鼠20只,取出40根小鼠股骨浸泡于戊二醛溶液1周,将小鼠股骨随机分为无水合制备组和水合制备组,每组20根。无水合制备组于扫描当天直接从戊二醛溶液中取出离体小鼠股骨,扫描过程中采用直接暴露在空气中。水合制备组于扫描前1天将固定于戊二醛溶液中的离体小鼠股骨放入EP管中,加入纯净水进行12 h水合,扫描过程中内部采用湿润纸巾包裹,外边再包保鲜膜,模拟小鼠股骨在活体的环境下进行显微CT(Mirco-CT)扫描。两组采用完全一致的CT扫描参数,进行重建与分析,对比两组BMD和TMD检测结果。结果 水合制备组BMD检测结果高于无水合制备组,差异有统计学意义(P<0.05);两组TMD检测结果比较,差异无统计学意义(P>0.05)。结论 无水合制备法直接影响了BMD的测量结果准确性,对TMD的测量结果产生的影响较小,使用水合制备法更能保证实验结果的准确性。Objective To explore the effect of different sample preparation methods on CT quantitative measurement of trabecula bone mineral density(BMD) and cortical bone tissue mineral DENSITY(TMD) in isolated femur of mice.Methods Twenty female BALA/C mice from 6 to 8 weeks were taken out and soaked in glutaraldehyde solution for 1 week.The femurs of mice were randomly divided into two groups:non-hydration preparation group and hydration preparation group,with 20 femurs in each group.In the non-hydrated preparation group,isolated mouse femurs were directly removed from glutaraldehyde solution on the day of scanning,and exposed to air during scanning.In the hydration preparation group,the isolated mouse femur fixed in glutaraldehyde solution was placed into EP tube one day before the scanning,and purified water was added for hydration for 12h.During the scanning process,the inside of the femur was wrapped with moist paper towels,and the outside was wrapped with plastic film.Micro-ct(mirco-CT) scanning was performed to simulate the femur of mice in vivo.The two groups were reconstructed and analyzed using CT scan parameters that were completely consistent,and BMD and TMD detection results were compared between the two groups.Results BMD test results of hydration group were higher than those of non-hydration group,and the difference was statistically significant( P<0.05).There was no significant difference in TMD test results between the two groups(P >0.05).Conclusion Anhydrous preparation method directly affects the accuracy of BMD measurement results,and has little effect on TMD measurement results.Hydration preparation method can ensure the accuracy of experimental results.

关 键 词：Mirco-CT 小鼠股骨 骨小梁密度 骨皮质密度 水合制备 

分 类 号：R68[医药卫生—骨科学]