2种唐松草属植物异喹啉类生物碱的研究  

Isoquinoline alkaloids from two species of Thalictrum genus plants

作　　者：薛晶晶 李建勇 李冰洁 金洋涛 王朝慧 薛春梅 张红梅 李占林 李达翃 华会明 XUE Jing-jing;LI Jian-yong;LI Bing-jie;JIN Yang-tao;WANG Chao-hui;XUE Chun-mei;ZHANG Hong-mei;LI Zhan-lin;LI Da-hong;HUA Hui-ming(Key Laboratory of Structure-Based Drug Design&Discovery,Ministry of Education,School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China)

机构地区：[1]沈阳药科大学中药学院基于靶点的药物设计与研究教育部重点实验室,辽宁沈阳110016

出　　处：《中国中药杂志》2022年第10期2676-2680,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(82173715);国家“重大新药创制”科技重大专项(2018ZX09735005);沈阳药科大学创新创业训练计划项目(202010163012)。

摘　　要：该文对2种唐松草属植物高原唐松草Thalictrum cultratum和贝加尔唐松草T.baicalense根的化学成分进行了研究。通过硅胶、氧化铝、ODS、Sephadex LH-20凝胶等多种柱色谱方法,以及制备高效液相等分离手段,从2种唐松草属植物乙醇提取物中分离鉴定了11个简单异喹啉类生物碱,包括1个新化合物,命名为去氢黄白蓬草碱(1),和10个已知化合物:N-甲基紫堇碱(2)、N-methylthalidaldine(3)、黄白蓬草碱(4)、氧化北美黄连次碱(5)、降氧化北美黄连次碱(6)、6,7-二甲氧基-2-甲基异喹诺酮(7)、thalactamine(8)、去氢降氧化北美黄连次碱(9)、6,7-二甲氧基-2-甲基-1,2,3,4-四氢异喹啉(10)、isopicnarrhine(11)。通过HR-ESI-MS、1D和2D NMR等波谱技术鉴定了化合物的结构。其中化合物1为新的简单异喹啉类生物碱,化合物11为首次从该属植物中分离得到。对化合物的细胞毒活性进行了评价,实验结果表明所有化合物对肿瘤细胞株HL-60、U937、HCT116、Caco-2、HepG2均无明显的细胞毒活性。The chemical constituents from the roots of Thalictrum cultratum and T.baicalense were investigated.By various isolation methods,such as silica gel,aluminium oxide,ODS,and Sephadex LH-20 column chromatographies,and semi-preparative HPLC,11 simple isoquinoline alkaloids were isolated from the ethanol extract of the roots of these two plants,including a new compound,named dehydrothalflavine(1),and ten known ones(2-11):N-methylcorydaline(2),N-methylthalidaldine(3),thaliflavine(4),oxyhydrastinine(5),noroxyhydrastinine(6),dimethoxyisoquinolone(7),thalactamine(8),dehydronoroxyhydrastinine(9),6,7-dimethoxy-2-methyl-1,2,3,4-tetrahydroisoquinoline(10),and isopicnarrhine(11).Their structures were elucidated on the basis of HR-ESI-MS and 1 D and 2 D NMR techniques.Compound 1 was a new isoquinoline alkaloid.Compound 11 was obtained from Tha-lictrum plant for the first time.All compounds did not show cytotoxic activities against HL-60,U937,HCT116,Caco-2,and HepG2 cancer cell lines.

关 键 词：高原唐松草 贝加尔唐松草 简单异喹啉类生物碱 细胞毒活性 

分 类 号：R284.1[医药卫生—中药学]