醋甘遂成分群B、C对Walker-256癌性腹水模型大鼠肠道菌群多样性的影响  

Chemical ingredient groups B and C in Kansui Radix stir-fried with vinegar affect diversity of gut microbiota in rat model of malignant ascites induced by Walker-256 cells

作　　者：张毅[2] 姜东京 周世康 王旻宇 张丽 ZHANG Yi;JIANG Dong-jing;ZHOU Shi-kang;WANG Min-yu;ZHANG Li(Nanjing University of Chinese Medicine Hanlin College,Taizhou 225300,China;Jiangsu Provincial Collaborative Innovation Center for Industrialization Process of Traditional Chinese MedicineResources/National-Local Joint Engineering Research Center for Traditional Chinese Medicine Resources Industrialization,Prescriptions and Innovative Drugs,Nanjing University of Chinese Medicine,Nanjing 210023,China;Nanjing University of Chinese Medicine Hanlin College,Suzhou 215000,China)

机构地区：[1]南京中医药大学翰林学院,江苏泰州225300 [2]南京中医药大学江苏省中药资源产业化过程协同创新中心中药资源产业化与方剂创新药物国家地方联合工程研究中心,江苏南京210023 [3]苏州卫生职业技术学院药学院,江苏苏州215000

出　　处：《中国中药杂志》2022年第5期1253-1261,共9页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81673599);江苏省333工程人才项目(BRA2020071);江苏省自然科学基金项目(BK20201232)。

摘　　要：探讨醋甘遂成分群B、C对腹水模型大鼠肠道菌群多样性的影响,找寻与其泻水逐饮作用相关的关键差异性菌群,揭示相关成分群泻水逐饮的生物学机制。以Walker-256癌性腹水模型大鼠为研究对象,果导片为阳性药,分组连续7 d灌胃醋甘遂成分群B、C。实验第6天灌胃后,收集新鲜粪便样品,通过16S rDNA高通量测序技术及GC-MS定量分析粪便短链脂肪酸的方法研究醋甘遂成分群B、C对模型大鼠肠道菌群的调节作用。实验第7天灌胃后,采集血清和肠组织样本,测定相关指标。结果显示,与空白组比较,模型组大鼠排便量、尿量显著降低(P<0.01),腹水量、尿Na^(+)、K^(+)、Cl^(-)水平显著增加(P<0.01),肠AQP8及其mRNA表达降低(P<0.01)。大鼠肠道有益菌乳杆菌属的丰度降低(P<0.01),而潜在致病菌毛螺菌科、厌氧支原体属等丰度均升高(P<0.01),短链脂肪酸含量显著降低(P<0.01)。与模型组比较,模型醋B组、模型醋C组大鼠的上述指标均有不同程度回调(P<0.01)。推知醋甘遂成分群B、C可通过增加模型大鼠有益菌乳杆菌属的丰度,降低有害菌毛螺菌属和厌氧支原体属的丰度,调节腹水大鼠紊乱的肠道菌,发挥泻下逐水作用。该研究可为醋甘遂临床合理应用提供参考。This study aims to explore the effects of chemical ingredient groups B and C in Kansui Radix stir-fried with vinegar on the diversity of gut microbiota in the rat model of malignant ascites,identify the key differential microbial taxa,and reveal the biological mechanism of water-expelling effect of the two chemical ingredient groups.The rat model of malignant ascites induced by Walker-256 cells was established,and phenolphthalein was used as the positive drug.The rats were orally administrated with corresponding agents for consecutive 7 days.On day 6,fresh feces samples were collected from the rats,and 16 S rDNA high-throughput sequencing and GC-MS were employed to determine the composition of gut microbiota and the content of short-chain fatty acids,respectively.On day 7,serum and intestinal tissue samples were collected for the determination of related indicators.Compared with the control group,the model group showed decreased feces volume and urine volume(P<0.01),increased volume of ascites and levels of Na^(+),K^(+),and Cl^(-)in urine(P<0.01),down-regulated mRNA and protein levels of intestinal AQP8(P<0.01),lowered abundance of beneficial Lactobacillus(P<0.01)while risen abundance of potential pathogenic Lachnospiraceae and Anaeroplasma(P<0.01),and reduced content of short-chain fatty acids(P<0.01).Compared with the model group,administration with chemical ingredient groups B and C alleviated all the above indicators(P<0.01).In conclusion,chemical ingredient groups B and C in Kansui Radix stir-fried with vinegar could alleviate the disordered gut microbiota in rats with malignant ascites to expel water through increasing the abundance of beneficial Lactobacillus and reducing the abundance of harmful Lachnospiraceae and Anaeroplasma.This study can provide a reference for the reasonable clinical application of Kansui Radix stir-fried with vinegar.

关 键 词：醋甘遂 成分群 肠道菌群 癌性腹水 泻水逐饮 

分 类 号：R285.5[医药卫生—中药学]