黄连内生真菌Aspergillus sp.ZJ-58中1个新的聚酮类化合物  

A new polyketide of endophytic fungi Aspergillus sp.ZJ-58 from Coptis chinensis

作　　者：尹国平 文淳 李亚娟 石牍 朱晶晶[2] 胡昌华[1] YIN Guo-ping;WEN Chun;LI Ya-juan;SHI Du;ZHU Jing-jing;HU Chang-hua(Engineering Research Center for Development and Utilization of Coptis Chinensis,College of Pharmaceutical Sciences,Southwest University,Chongqing 400716,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Jiulongpo District Hospital of Traditional Chinese Medicine,Chongqing 400050,China)

机构地区：[1]西南大学药学院,黄连开发与利用教育部工程研究中心,重庆400716 [2]中国中医科学院中药研究所,北京100700 [3]重庆市九龙坡区中医院,重庆400050

出　　处：《中国中药杂志》2022年第4期967-971,共5页China Journal of Chinese Materia Medica

基　　金：国家重点研发计划项目(2017YFC170260103);重庆市卫生计生委中医药科技项目(ZY201802134);国家自然科学基金项目(82003629)。

摘　　要：针对重庆道地药材黄连,进行内生真菌分离,获得1株曲霉属真菌。通过大米固体发酵,利用硅胶、MCI、HPLC半制备等色谱分离方法进行分离纯化,运用MS、NMR、IR、UV、ECD等方法对其进行结构确证,共分离鉴定了3个化合物,包括2个聚酮类化合物coptaspin A(1)、4-acetyl-3,4-dihydro-6,8-dihydroxy-3-methoxy-5-methylisocoumarin(2),和1个细胞松弛素类化合物cytochalasin Z_(12)(3)。其中,化合物1为新化合物。活性试验表明,化合物1对脂多糖诱导的小鼠单核巨噬细胞释放一氧化氮具有抑制活性,其IC_(50)为58.7μmol·L^(-1),显示出潜在的抗炎活性。A new polyketide,coptaspin A(1),along with two known compounds 4-acetyl-3,4-dihydro-6,8-dihydroxy-3-methoxy-5-methylisocoumarin(2),and cytochalasin Z_(12)(3),was isolated from the endophytic fungi Aspergillus sp.ZJ-58,which was isolated from the genuine medicinal plant Coptis chinensis in Chongqing after solid-state fermentation on rice and silica gel,MCI,and HPLC-based separation.Their structures were elucidated by MS,NMR,IR,UV,and ECD.The newly isolated compound 1 showed moderate inhibitory activities against LPS-induced NO production in RAW264.7 macrophages with the IC_(50)value of 58.7μmol·L^(-1),suggesting its potential anti-inflammatory activity.

关 键 词：黄连 内生真菌 coptaspin A 抗炎活性 

分 类 号：R284.1[医药卫生—中药学]