基于血清代谢组学的藏族药翼首草提取物治疗类风湿性关节炎的作用机制研究  

Mechanism of Tibetan medicine Pterocephalus hookeri extract in treatment of rheumatoid arthritis based on serum metabonomics

作　　者：唐策[1,2] 干志强 罗世英 杨娟[3] 于猛[4] 邹忠梅[4] 张艺[1,2] TANG Ce;GAN Zhi-qiang;LUO Shi-ying;YANG Juan;YU Meng;ZOU Zhong-mei;ZHANG Yi(School of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Ethnic Medicine Academic Heritage Innovation Research Center,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China)

机构地区：[1]成都中医药大学民族医药学院,四川成都611137 [2]成都中医药大学民族医药文化传承创新研究中心,四川成都611137 [3]成都中医药大学药学院,四川成都611137 [4]中国医学科学院,北京协和医学院,药用植物研究所,北京100193

出　　处：《中国中药杂志》2022年第4期1001-1008,共8页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81903922,81274193);成都中医药大学“杏林学者”潜质博士后项目(BSH2019002);国家重点研发计划项目(2017YFC1703900)。

摘　　要：该研究基于超高效液相色谱-四级杆-飞行时间串联质谱(UPLC-Q-TOF/MS)探讨藏族药翼首草对完全弗氏佐剂诱发的佐剂性关节炎(AA)模型大鼠血清代谢组的影响。AA模型造模成功后,给药30 d,取大鼠血清,处理后采集UPLC-Q-TOF-MS谱,并用主成分分析(PCA)、正交偏最小二乘判别分析(OPLS-DA)的方法对其进行分析。结果显示,与对照组比较,模型组大鼠血清中12个生物标志物含量均显著升高(P<0.05),而其他9个生物标志物含量显著降低(P<0.05);翼首草提取物给药组可使上述21个中的19个生物标志物回调到一定范围;通路富集显示,这些标志物主要涉及缬氨酸、亮氨酸和异亮氨酸降解,花生四烯酸代谢,精氨酸和脯氨酸代谢,甘油磷脂代谢,初级胆汁酸生物合成,胆汁酸生物合成,色氨酸代谢,不饱和脂肪酸生物合成等8条代谢途经。结果表明翼首草提取物可以调节代谢紊乱,促进新陈代谢表型向正常水平回归,对佐剂性关节炎大鼠具有治疗作用,为翼首草治疗类风湿性关节炎的生物学基础研究提供了一定的科学依据。Ultra-high performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry(UPLC-Q-TOF/MS)was used to investigate the effect of Pterocephalus hookeri on serum metabolism of adjuvant arthritis(AA)model rats induced by complete Freund′s adjuvant.After the AA model was properly induced,the serum of rats was collected 30 days after treatment.UPLC-Q-TOF-MS chromatograms were collected and analyzed by principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA).The results revealed that compared with the control group,the model group showed increased content of 12 biomarkers in the serum(P<0.05)and reduced content of the other nine biomarkers(P<0.05).P.hookeri extract could recover the above-mentioned 19 biomarkers to a certain range.Pathway enrichment showed that these markers mainly involved eight metabolic pathways,including valine,leucine,and isoleucine degradation,arachidonic acid metabolism,arginine and proline metabolism,glycerol phospholipid metabolism,primary bile acid biosynthesis,bile acid biosynthesis,tryptophan metabolism,and unsaturated fatty acid biosynthesis.The findings of this study demonstrate that P.hookeri extract can regulate metabolic disorders and promote the regression of metabolic phenotype to the normal level to exert the therapeutic effect on AA rats.This study is expected to provide a certain scientific basis for the biological research on the treatment of rheumatoid arthritis by P.hookeri.

关 键 词：血清代谢组学 藏族药 翼首草 类风湿性关节炎 超高效液相色谱-四级杆-飞行时间串联质谱(UPLC-Q-TOF-MS) 

分 类 号：R29[医药卫生—民族医学]