川白芷MYB-related家族的生物信息及表达模式分析  

Bioinformatics and expression analysis on MYB-related family in Angelica dahurica var. formosana

作　　者：姚菲 江美彦 杨云舒 杨雪梅 杜宣 刘仁浪 侯凯[1] 吴卫[1] YAO Fei;JIANG Mei-yan;YANG Yun-shu;YANG Xue-mei;DU Xuan;LIU Ren-lang;HOU Kai;WU Wei(College of Agronomy,Sichuan Agricultural University,Wenjiang 611130,China)

机构地区：[1]四川农业大学农学院,四川温江611130

出　　处：《中国中药杂志》2022年第7期1831-1846,共16页China Journal of Chinese Materia Medica

基　　金：四川省科技厅重点研发项目(2021YFYZ0012);川渝联合实施重点研发项目(2020YFQ0054);四川省中医药重点学科建设项目(2021-16-4)。

摘　　要：近年来,研究发现MYB-related家族基因在植物的生长发育过程中起着重要作用。该文以“川芷2号”为供试材料,运用转录组数据库检索和生物信息学的方法系统分析白芷MYB-related家族基因,并通过实时荧光定量PCR对其时空表达特性进行分析。研究表明,共鉴定出122个MYB-related家族蛋白,主要为热稳定性良好的不稳定性亲水蛋白;大部分蛋白定位于细胞核中;该家族蛋白无规卷曲和α-螺旋所占比例较大;5个白芷MYB-related家族蛋白具有膜结合结构域;通过对白芷MYB-related家族蛋白保守结构域分析,5个亚组基因的MYB结构域与2R-、3R-和4R-MYB蛋白类似,含有MYB重复序列中3个均匀分布的Trp(W)残基。通过白芷和拟南芥MYB-related家族蛋白的系统进化分析显示,二者的MYB-related成员在5个亚组中均有不均匀分布,且在CCA1-like亚组中拟南芥和白芷具有几乎相同的基因数量;122条编码蛋白所含基序数量、类型、分布上存在差异,分支相近的转录因子具有相似的结构域及基序;表达模式分析显示AdMYB53、AdMYB83、AdMYB89转录因子对激素均有不同程度响应,且均在叶中表达量较高,根部响应迅速。该研究为进一步研究白芷MYB-related转录因子的功能及解决白芷早抽薹等生物学问题奠定了基础。In recent years, the MYB-related gene family has been found pivotal in plant growth and development. MYB-related gene family in Angelica dahurica var. formosana was systematically investigated based on "Chuanzhi No. 2" through transcriptome database search and bioinformatics and the temporal and spatial expression patterns were analyzed through real-time fluorescence-based quantitative polymerase chain reaction(PCR). The results showed that 122 MYB-related proteins family were identified, mainly including the unstable hydrophilic proteins with good thermal stability. Most of the proteins were located in nuclei. The majority of the proteins had the structures of random coil and α-helix. Five MYB-related proteins family of A. dahurica var. formosana had membrane-binding domains. The conserved domain analysis of MYB-related proteins family of A. dahurica var. formosana showed that the MYB domains of genes in five subgroups, similar to 2 R-, 3 R-, and 4 R-MYB proteins, contained three evenly distributed Trp(W) residues in the MYB repeat sequence. The phylogenetic analysis of MYB-related proteins family in A. dahurica var. formosana and Arabidopsis thaliana showed that the MYB-related members were unevenly distributed in five subgroups, and A. thaliana and A. dahurica var. formosana had almost the same number of genes in the CCA1-like subgroup. There were differences in the number, type, and distribution of motifs contained in 122 encoded proteins. Transcription factors with similar branches had similar domains and motifs. The expression pattern analysis showed that the transcription factors AdMYB53, AdMYB83, and AdMYB89 responded to hormones to varying degrees, and they were highly expressed in leaves and responded quickly in roots. This study lays a foundation for further investigating the function of MYB-related transcription factors of A. dahurica var. formosana and solving the corresponding biological problems such as bolting early.

关 键 词：白芷 MYB-related家族 生物信息学分析 表达分析 

分 类 号：S567.239[农业科学—中草药栽培]