黄连内生真菌炭角菌次生代谢产物研究  

Secondary metabolites of endophyte fungi Xylaria sp.from Coptis chinensis

作　　者：尹国平 李亚娟 李博 刘雪梅[1] 朱晶晶[2] 王智民[2] 胡昌华[1] YIN Guo-ping;LI Ya-juan;LI Bo;LIU Xue-mei;ZHU Jing-jing;WANG Zhi-min;HU Chang-hua(Engineering Research Center of Coptis Development and Utilization(Ministry of Education),College of Pharmaceutical Sciences,Southwest University,Chongqing 400716,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]西南大学药学院黄连开发与利用教育部工程研究中心,重庆400716 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国中药杂志》2022年第8期2165-2169,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(82003629);国家重点研发计划项目(2017YFC170260103);大学生创新创业训练计划项目(202110635104)。

摘　　要：针对重庆道地药材黄连,进行内生真菌分离,获得一株炭角菌属真菌。通过大米固体发酵,利用硅胶、MCI、HPLC半制备等色谱分离方法进行分离纯化,运用MS、NMR、IR、UV、ECD等方法进行结构确证。共分离鉴定了5个化合物,分别为lasobutone A(1)、lasobutone B(2)、guignardianone C(3)、guignardic acid(4)和4-hydroxy-17R-methylincisterol(5)。其中,化合物1和2为新化合物。活性测试表明,化合物2和4对脂多糖诱导的小鼠单核巨噬细胞释放一氧化氮具有一定抑制活性,其IC;分别为58.7、42.5μmol·L^(-1);化合物5对结肠癌细胞HT-29具出显著的抑制活性,IC;为14.3μmol·L^(-1)。Two new polyketides,lasobutone A(1)and lasobutone B(2),along with three known compounds,guignardianone C(3),guignardic acid(4),and 4-hydroxy-17R-methylincisterol(5),were isolated from the endophytic fungi Xylaria sp.by silica gel,MCI,and preparative HPLC,which was separated from the Chinese medicinal material Coptis chinensis and cultivated through solid fermentation with rice.Their structures were elucidated on the basis of spectroscopic methods,such as MS,NMR,IR,UV,and ECD.Compounds 2 and 4 showed inhibitory activities against the nitric oxide(NO)production in the LPS-induced macrophage RAW264.7 with IC;values of 58.7 and 42.5μmol·L^(-1)respectively,while compound 5 exhibited cytotoxic activities against HT-29 with IC;value of 14.3μmol·L^(-1).

关 键 词：黄连 炭角菌 lasobutone A lasobutone B 

分 类 号：R284[医药卫生—中药学]