赤红球菌甲苯胁迫响应蛋白基因的克隆、生物信息学分析和表达变化研究  

The Cloning,Bioinformatic Analysis and Expression Variation of a Gene Cloning Toluene Stress Response Protein in Rhodoccocus ruber

作　　者：张帆 袁媛 曾艳 付薇 彭仁[1] ZHANG Fan;YUAN Yuan;ZENG Yan;FU Wei;PENG Ren(College of Life Science,Jiangxi Normal University,Nanchang Jiangxi 330022,China)

机构地区：[1]江西师范大学生命科学学院,江西南昌330022

出　　处：《江西师范大学学报（自然科学版）》2022年第2期140-146,共7页Journal of Jiangxi Normal University(Natural Science Edition)

基　　金：国家自然科学基金(31960011);江西省研究生创新专项资金(YC2020-S185)资助项目.

摘　　要：从赤红球菌SD3中克隆了一个甲苯胁迫响应蛋白基因tsrp1,该基因长度为417 bp,在GenBank的登录号为MK371001.该基因编码的蛋白质Tsrp1由138个氨基酸残基组成,理论分子量为14.2 kDa,理论等电点为4.82,不稳定系数为18.68,总平均疏水指数为-0.154,属于酸性稳定的亲水蛋白.Tsrp1无信号肽和二硫键、无跨膜区、无保守结构域,存在于细胞质中.在R.ruber SD3中Tsrp1蛋白与R.rhodochrous、R.zopfii、R.pyridinivorans、R.hoagii、M.brevis和C.manganitolerans中的序列相似蛋白的相似性分别为94.93%、71.29%、67.96%、44.58%、42.05%和35.00%.通过分子对接发现Tsrp1与c-di-GMP存在相互作用.在甲苯和苯酚胁迫下,在赤红球菌SD3中tsrp1基因的表达分别为原来的40.19倍和14.73倍.Agene of tsrp1 encoding toluene stress response protein is cloned from Rhodoccocus ruber SD3.The length of the gene is 417 bp with the accession number of MK371001 in GenBank.It encodes a protein of 138 amino acid residues with an estimated molecular weight of 14.2 kD and an isoelectric point of 4.82.It also has a instability index of 18.68 and a grand average of hydropathicity of-0.154,and thus it is acidic,stable and hydrophilic protein.The protein has no signal peptide,disulfide bonds and conservative domain.It exists in the cytoplasm.Tsrp1 in Rhodoccocus ruber SD3 has a similarity of 94.93%,71.29%,67.96%,44.58%,42.05%and 35.00%with sequence similar proteins in R.rhodochrous,R.zopfii,R.pyridinivorans,R.hoagii,M.brevis and C.manganitolerans,respectively.Tsrp1 is found to interact with c-di-GMP by molecular docking.The expression of tsrp1 gene has an increase of 40.19 folds and 14.73 folds under toluene and phenol stress,respectively.

关 键 词：赤红球菌 有机溶剂胁迫 荧光定量PCR 生物信息学 

分 类 号：Q89[生物学]