Identification of candidate genes related to soluble sugar contents in soybean seeds using multiple genetic analyses  

作　　者：PAN Wen-jing HAN Xue HUANG Shi-yu YU Jing-yao ZHAO Ying QU Ke-xin ZHANG Ze-xin YIN Zhen-gong QI Hui-dong YU Guo-long ZHANG Yong XIN Da-wei ZHU Rong-sheng LIU Chun-yan WU Xiao-xia JIANG Hong-wei HU Zhen-bang ZUO Yu-hu CHEN Qing-shan QI Zhao-ming 

机构地区：[1]College of Agriculture,Northeast Agricultural University,Harbin 150030,P.R.China [2]Heilongjiang Bayi Agricultural University,Daqing 163000,P.R.China [3]Keshan Branch of Heilongjiang Academy of Agricultural Sciences,Qiqihar 161300,P.R.China

出　　处：《Journal of Integrative Agriculture》2022年第7期1886-1902,共17页农业科学学报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(31701449,31971968,31971899,and 31501332);the Natural Science Foundation of Heilongjiang,China(QC2017013);the National Key R&D Program of China(2016YFD0100500,2016YFD0100300 and 2016YFD0100201-21);the Special Financial Aid to PostDoctor Research Fellow in Heilongjiang,China(LBHTZ1714);the International Postdoctoral Exchange Fellowship Program of China Postdoctoral Council(20180004);the China Post Doctoral Project,China(2015M581419);the Post-Doctoral Project of Northeast Agricultural University,China(NEAUBH-19002);the Heilongjiang Funds for Distinguished Young Scientists,China(JC2016004 and JC2017006);the Dongnongxuezhe Project,China(to Chen Qingshan);the the Backbone of Young Talent Scholar Project(to Qi Zhaoming,18XG01)of Northeast Agricultural University,China。

摘　　要：Soluble sugar content in seeds is an important quality trait of soybean. In this study, 57 quantitative trait loci(QTLs) related to soluble sugar contents in soybean seeds were collected from databases and published papers. After meta-overview-collinearity integrated analysis to refine QTL intervals, eight consensus QTLs were identified. To further verify the consensus QTLs, a population of chromosome segment substitution lines(CSSLs) was analyzed. Two lines containing fragments covering the regions of consensus QTLs and the recurrent parent were selected: one line showed high soluble sugar contents associated with a consensus QTL fragment, and the other line showed low soluble sugar contents. Transcriptome sequencing was conducted for these two lines at the early, middle, and late stages of seed development, which identified 158, 109 and 329 differentially expressed genes, respectively. Based on the analyses of re-sequencing data of the CSSLs and the consensus QTL region, three candidate genes(Glyma.19 G146800, Glyma.19 G122500, and Glyma.19 G128500) were identified in the genetic fragments introduced from wild soybean. Sequence comparisons between the two CSSL parents SN14 and ZYD00006 revealed a single nucleotide polymorphism(SNP) mutation in the coding sequence of Glyma.19 G122500, causing a nonsynonymous mutation in the amino acid sequence that affected the predicted protein structure. A Kompetitive allele-specific PCR(KASP) marker was developed based on this SNP and used to evaluate the CSSLs. These results lay the foundation for further research to identify genes related to soluble sugar contents in soybean seeds and for future soybean breeding.

关 键 词：soybean soluble sugar contents consensus QTL meta-overview-collinearity integrated analysis population validation RNA-seq and candidate gene mining 

分 类 号：S565.1[农业科学—作物学]