Overexpression of MdMIPS1 enhances drought tolerance and water-use efficiency in apple  

作　　者：HU Ling-yu YUE Hong ZHANG Jing-yun LI Yang-tian-su GONG Xiao-qing ZHOU Kun MA Feng-wang 

机构地区：[1]State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple,College of Horticulture,Northwest A&F University,Yang ling 712100,P.R.China

出　　处：《Journal of Integrative Agriculture》2022年第7期1968-1981,共14页农业科学学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2018YFD1000303);the China Agriculture Research System of MOF and MARA(CARS-27)。

摘　　要：Myo-inositol and its derivatives play important roles in the tolerance of higher plants to abiotic stresses, and myo-inositol-1-phosphate synthase(MIPS) is the rate-limiting enzyme in myo-inositol biosynthesis. In this study, we found that increased myo-inositol biosynthesis enhanced drought tolerance in MdMIPS1-overexpressing apple lines under shortterm progressive drought stress. The effect of myo-inositol appeared to be mediated by the increased accumulation of osmoprotectants such as glucose, sucrose, and proline, and by the increased activities of antioxidant enzymes that eliminate reactive oxygen species. Moreover, enhanced water-use efficiency(WUE) was observed in MdMIPS1-overexpressing apple lines under long-term moderate water deficit conditions that mimicked the water availability in the soil of the Loess Plateau. Enhanced WUE may have been associated with the synergistic regulation of osmotic balance and stomatal aperture mediated by increased myo-inositol biosynthesis. Taken together, our findings shed light on the positive effects of MdMIPS1-mediated myo-inositol biosynthesis on drought tolerance and WUE in apple.

关 键 词：APPLE MdMIPS1 MYO-INOSITOL water deficit drought tolerance water-use efficiency 

分 类 号：S661.1[农业科学—果树学]