泰州市55~65岁汉族农村居民肠道菌群与骨密度关联性分析  

Association of gut microbiota with bone mineral density in 55-65 years old rural Han residents of Taizhou city

作　　者：杨丹桐 朱嗣博[1,2,3] 蒋艳峰 田为中[4] 张德坤 陈锦华[4] 范敏[6] 崔梅 徐珂琳[2,8] 陈兴栋 YANG Dan-tong;ZHU Si-bo;JIANG Yan-feng(State Key Laboratory of Genetic Engineering,Human Phenome Institute,Fudan University,Shanghai 200000 China;不详)

机构地区：[1]复旦大学生命科学学院人类学教育部重点实验室生命科学学院,上海200438 [2]复旦大学泰州健康科学研究院 [3]复旦大学公共卫生学院流行病学教研室公共卫生安全教育部重点实验室 [4]南通大学附属泰州市人民医院医学影像科 [5]泰州市疾病预防控制中心 [6]泰兴市疾病预防控制中心 [7]复旦大学附属华山医院神经内科 [8]复旦大学公共卫生学院生物统计学系公共卫生安全教育部重点实验室

出　　处：《中国公共卫生》2022年第6期743-748,共6页Chinese Journal of Public Health

基　　金：国家重点研发计划项目(2017YFC0907500,2017YFC0907000);上海市扬帆计划项目(19YF1403400);上海市科技重大专项(2017SHZDZX01);中国博士后科学基金项目(2019M661376)。

摘　　要：目的了解江苏省泰州市55~65岁汉族农村居民肠道菌群与骨密度之间的关联性,为骨质疏松症的预防提供新的思路。方法于2013年3月—2018年10月采用整群随机抽样方法抽取泰州队列中依从性和应答率较高的3个自然村中537名55~65岁在泰州市居住≥20年的汉族农村居民进行问卷调查、体格检查、骨密度测量和粪便检查,并参照世界卫生组织的诊断标准将其分为正常骨密度组、低骨量组和骨质疏松组3组,分析组间菌群的差异以及肠道菌群与骨密度之间的相关性。结果泰州市537名55~65岁汉族农村居民中,正常骨密度组207人(38.55%),低骨量组239人(44.51%),骨质疏松组91例(16.94%)。在校正了性别、年龄、体质指数、吸烟情况和饮酒情况等变量后,偏相关分析结果显示,新鲜蔬菜(β=-0.125)、新鲜水果(β=-0.101)、肉类(β=-0.098)和酸奶(β=-0.033)摄入频次均与骨密度分组呈负相关(均P<0.05)。与正常骨密度组比较,梭形杆菌、布劳特氏菌、弯曲杆菌、大肠杆菌、链球菌、放线菌、志贺氏菌、厌氧菌、链霉菌和奇异果菌在低骨量组和骨质疏松组的丰度均有所下降,而棒状杆菌、韦洛内拉菌和短链单胞菌在低骨量组和骨质疏松组的丰度则均有所增加(均P<0.05)。在调整了性别、年龄、体质指数、吸烟情况、饮酒情况、新鲜蔬菜摄入频次、新鲜水果摄入频次、肉类摄入频次和酸奶摄入频次等混杂因素后,多因素非条件logistic回归分析结果显示,大肠杆菌(OR=0.992,95%CI=0.985~0.999)、志贺氏菌(OR=0.984,95%CI=0.970~0.998)、布劳特氏菌(OR=0.959,95%CI=0.924~0.996)和镰刀菌(OR=0.980,95%CI=0.962~0.998)丰度的增加是泰州市55~65岁汉族农村居民低骨量发生的保护因素,粪杆菌丰度的增加(OR=1.060,95%CI=1.006~1.118)是泰州市55~65岁汉族农村居民骨质疏松发生的危险因素,木杆菌丰度的增加(OR=0.865,95%CI=0.763~0.981)是泰州市55~65岁汉族农村�Objective To examine the relationship between intestinal flora and bone mineral density among rural Han residents aged 55-65 years in Taizhou city,Jiangsu province for exploring novel approaches to osteoporosis prevention.Methods Using cluster random sampling,we recruited 537 rural Han long term(≥20 years)residents aged 55-65 years in 3 villages with more compliant followees and high response rate in a cohort for multidisciplinary and multiple diseases research-the Taizhou Longitudinal Study.Questionnaire interview,physical examination,bone density measurement and stool specimen detection were carried out among the selected residents from March 2013 through October 2018.The residents were then assigned into one of the 3 groups with normal bone density,osteopenia and osteoporosis based on bone density measurement according to the diagnostic criteria of World Health Organization.Differences in intestinal flora among the 3 groups and the correlation between intestinal flora and bone density were analyzed.Results Of all the participants,38.55%(207),44.51%(239),and 16.94%(91)were assessed with normal bone density,osteopenia,and osteoporosis,respectively.After adjusting for variables such as gender,age,body mass index(BMI),smoking and alcohol drinking,the results of partial correlation analysis showed that intake frequency of fresh vegetables(β=-0.125),fresh fruits(β=-0.101),meat(β=-0.098)and yogurt(β=-0.033)were reversely correlated significantly with bone mineral density grouping(all P<0.05).Compared with those in the normal bone density group,the intestinal abundances of Clostridium,Blautia,Campylobacter,Escherichia,Streptococcus,Actinomyces,Shigella,Anaerostipes,Streptomyces and Atopobium were significantly decreased in both osteopenia and osteoporosis group;but the intestinal abundances of Corynebacterium,Veillonella and Brevundimonas increased in osteopenia and osteoporosis group(all P<0.05).After adjusting for confounding factors such as gender,age,BMI,smoking,alcohol drinking,and intake frequency of fresh

关 键 词：骨密度 肠道菌群 关联性分析 农村居民 汉族 

分 类 号：R18[医药卫生—流行病学]