刺梨多糖RRTFP-2结构分析及类NGF神经营养活性研究  

Structure Analysis and NGF-Like Neurotrophic Activity of Polysaccharides RRTFP-2 from Rosa Roxburghii Tratt

作　　者：谢慧荣 邓靖[2] 杨小生 李立郎[2,3] 王瑜 李齐激[2,3] 罗忠圣 杨娟[2,3] XIE Huirong;DENG Jing;YANG Xiaosheng;LI Lilang;WANG Yu;LI Qiji;LUO Zhongsheng;YANG Juan(College of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guiyang 550014,China;State Key Laboratory for Effectiveness and Utilization of Medicinal Plants,Guizhou Medical University,Guiyang 550014,China)

机构地区：[1]贵州中医药大学药学院,贵州贵阳550025 [2]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550014 [3]贵州医科大学,省部共建药用植物功效与利用国家重点实验室,贵州贵阳550014

出　　处：《食品科技》2022年第4期224-230,共7页Food Science and Technology

基　　金：中央和省级农业生产发展(第三批农村产业革命刺梨产业发展)专项(黔财农[2019]261号);贵州省科技支撑计划项目(黔科合支撑[2020]1Y148号);贵州省发改委工程中心项目(黔财建[2019]303号);贵州省教育厅高等学校特色重点实验室项目(黔教合KY字[2020]018号)。

摘　　要：采用离子交换、分子凝胶柱层析对刺梨干果粗多糖进行纯化,对得到的纯多糖RRTFP-2单糖组成、分子质量、糖醛酸含量及糖醛酸种类、主链及支链的组成、糖残基连接方式进行分析;运用大鼠嗜铬细胞瘤细胞(PC12细胞)模型考察RRTFP-2类神经生长因子(NGF)活性。结果显示,RRTFP-2是由鼠李糖、阿拉伯糖、甘露糖、葡萄糖、半乳糖、葡萄糖醛酸和半乳糖醛酸组成,中性单糖摩尔比为1.0:6.5:1.1:1.2:16.1,糖醛酸含量为11.42%,分子质量为74330 u的结构复杂的酸性杂多糖;RRTFP-2表现出类NGF神经营养活性。实验结果为刺梨多糖结构的阐明及其资源新用途提供参考。The crude polysaccharides of Rosa roxburghii Tratt dried fruit were purified by ion exchange and molecular gel column chromatography.The monosaccharide composition,molecular weight,content and type of uronic acid,composition of main chain and branch chain,and linkage mode of sugar residues of the obtained polysaccharide RRTFP-2 were analyzed.The activity of RRTFP-2 nerve growth factors(NGF)in rat pheochromocytoma cells(PC12 cells)was investigated.RRTFP-2 was a complex acidic heteropolysaccharide consisting of rhamnose,arabinose,mannose,glucose,galactose,glucuronic acid and galacturonic acid.The neutral monosaccharide molar ratio was 1.1:6.5:1.1:1.2:1.2:16.1,the content of uronic acid was 11.42%,and the molecular weight was 74330 U.RRTFP-2 showed NGF-like neurotrophic activity.The results provide reference for elucidating the structure of Rosa roxburghii Tratt polysaccharide and its new uses.

关 键 词：刺梨多糖 分离纯化 结构分析 大鼠嗜铬细胞瘤细胞(PC12细胞) 类NGF神经营养活性 

分 类 号：R284.2[医药卫生—中药学]