火炭母水提物对金黄色葡萄球菌的抑菌机制研究  

Antibacterial Mechanism of Polygonum Chinense L.Aqueous Extract Against Staphylococcus Aureus

作　　者：宋姗姗 杨艾华 王小敏 王微微 SONG Shanshan;YANG Aihua;WANG Xiaomin;WANG Weiwei(College of Basic Medical Sciences,Zunyi Medical University,Zunyi 563000,China)

机构地区：[1]遵义医科大学基础医学院,贵州遵义563000

出　　处：《食品科技》2022年第4期255-260,共6页Food Science and Technology

基　　金：国家自然科学基金项目(31860706);贵州省科技计划项目(黔科合平台人才[2018]5772-005);遵义市科技局联合资金项目(遵市科合HZ字[2020]82号);遵义医科大学大学生创新创业训练计划项目(ZYDX2020109)。

摘　　要：为明确火炭母水提物对金黄色葡萄球菌的抑菌机制,开发植物源天然防腐剂,研究采用微量稀释法测定火炭母水提物对金黄色葡萄球菌的最低抑菌浓度(MIC),通过结晶紫染色方法分析火炭母水提物对金黄色葡萄球菌生物膜形成的影响,并通过测定培养液中细菌生长曲线以及胞外蛋白和碱性磷酸酶(AKP)含量的变化,探讨火炭母水提物对金黄色葡萄球菌细胞膜和细胞壁的影响。结果表明,火炭母水提物对金黄色葡萄球菌的MIC为12.5 mg/mL,经火炭母水提物作用后,金黄色葡萄球菌生物膜形成能力减弱,细菌培养液中胞外蛋白和AKP含量明显增加。由此推断出火炭母水提物是通过破坏金黄色葡萄球菌细胞壁和细胞膜的完整性抑制细菌生长并干扰其生物膜形成。In order to determine the antibacterial mechanism of Polygonum chinense L.aqueous extract against Staphylococcus aureus and promote the development of antimicrobials,the minimal inhibitory concentration(MIC)of Polygonum chinense L.aqueous extract aginst Staphylococcus aureus was determined by double dilution method.Then the effect of the extract against the formation of Staphylococcus aureus biofilm was tested by crystal violet staining.The changes of growth curve,extracellular protein and alkaline phosphatase(AKP)in Staphylococcus aureus culture were observed to investigate the effects of Polygonum chinense L.aqueous extract on the c ell membrane and c ell wall of Staphylococcus aureus.The results showed that the MIC of Polygonum chinense L.extract was 12.5 mg/mL.After the intervention of the extract,the ability of biofilm formation decreased.The contents of extracelluar protein and AKP in Staphylococcus aureus culture increased significantly by dealing with Polygonum chinense L.aqueous extract.It was concluded that Polygonum chinense L.aqueous extract inhibits bacterial growth and interferes with biofilm formation by destroying the integrity of cell wall and cell membrane of Staphylococcus aureus.

关 键 词：火炭母 金黄色葡萄球菌 细胞膜 细胞壁 抑菌机制 

分 类 号：R284.2[医药卫生—中药学]