菟丝子总黄酮对PCOS大鼠血清甲状腺激素、排卵障碍的影响及机制研究  

Effects of Total Flavonoids of Tusizi(Cuscutae Semen)on Serum Thyroid Hormone and Ovulation Disorder in PCOS Rats and Its Mechanism

作　　者：赵千影 汪栋材[1] 吴海滨[1] 林基伟 宋晓容[1] ZHAO Qian-ying;WANG Dong-cai;WU Hai-bin;LIN Ji-wei;SONG Xiao-rong(Shenzhen Hospital of Traditional Chinese Medicine,Shenzhen Guangdong 518000,China)

机构地区：[1]深圳市中医院,广东深圳518000

出　　处：《中医药导报》2022年第5期30-34,46,共6页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：深圳市卫生健康委员会医防融合中医药组项目(深卫健体改〔2019〕25号);深圳市卫计委重点学科建设项目(SZXJ2018007);广东省中医药局科研课题(20211325)。

摘　　要：目的:观察菟丝子总黄酮对多囊卵巢综合征(PCOS)大鼠血清甲状腺激素及排卵障碍的影响,并探索可能机制。方法:取45只SD大鼠,随机选取10只设为对照组,剩余大鼠建立PCOS模型,采用随机数字表法将31只造模成功大鼠分为PCOS组(11只)、干预组(10只)、联合组(10只)。联合组大鼠灌胃菟丝子总黄酮(200 mg/kg),尾静脉注射丝裂原活化蛋白激酶(MAPK)/细胞外信号调节激酶(ERK)通路激动剂表皮生长因子(EGF)(10μg/kg);干预组大鼠灌胃菟丝子总黄酮(200 mg/kg),尾静脉注射等量PBS;对照组、PCOS组大鼠灌胃等体积生理盐水,尾静脉注射等量PBS,1次/d,持续21 d。检测血清促甲状腺激素(TSH)、游离三碘甲腺原氨酸(FT_(3))、游离甲状腺素(FT_(4))、三碘甲腺原氨酸(T_(3))、甲状腺素(T_(4))、促卵泡生长激素(FSH)、黄体生成激素(LH)、雌二醇(E_(2))水平;检测血清空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR);HE染色观察排卵情况;Western blotting法检测卵巢组织p38 MAPK、p-p38 MAPK、ERK1/2、p-ERK1/2蛋白表达量。结果:与PCOS组比较,干预组大鼠血清TSH、LH、FINS、HOMA-IR水平、囊性扩张卵泡率、卵巢组织p-p38 MAPK/p38 MAPK、p-ERK1/2/ERK1/2降低,FT_(3)、FT_(4)、T_(3)、T_(4)、FSH、E_(2)水平及排卵率升高,平均排卵数增加(P<0.05);与干预组比较,联合组血清TSH、LH、FINS、HOMA-IR水平、囊性扩张卵泡率、卵巢组织p-p38 MAPK/p38 MAPK、p-ERK1/2/ERK1/2升高,FT_(3)、FT_(4)、T_(3)、T_(4)、FSH、E_(2)水平、排卵率降低,平均排卵数减少(P<0.05)。结论:菟丝子总黄酮可改善PCOS大鼠甲状腺激素、性激素水平,降低胰岛素抵抗,促进排卵。其作用机制可能与抑制MAPK/ERK通路有关。Objective:To observe the effect of total flavonoids of Tusizi(Cuscutae Semen)on serum thyroid hormones and ovulation disorders in rats with polycystic ovary syndrome(PCOS),and explore possible mechanisms.Methods:Totally ten of the 45 SD rats were randomly selected 10 as the control group,and the remaining rats were establish the PCOS model.Totally 31 successfully rats were divided into PCOS group(n=11),intervention group(n=10)and combination group(n=10)by random number table method.Rats in the combination group were given total flavonoids of Tusizi(200 mg/kg)by gavage,and injected mitogen activated protein kinase(MAPK)/extracellular signal regulated kinase(ERK)pathway agonist epidermal growth factor(EGF)(10μg/kg)into the tail vein.Rats in the intervention group were given otal flavonoids of Tusizi(200 mg/kg)by gavage,and the same amount of PBS was injected into the tail vein.Rats in the control group and PCOS group were intragastrically administered with an equal volume of normal saline,and an equal volume of PBS was injected into the tail vein.Once a day for 21 days.The levels of serum thyroid-stimulating hormone(TSH),free triiodothyronine(FT_(3)),free thyroxine(FT_(4)),triiodothyronine(T_(3)),thyroxine(T_(4)),follicle-stimulating hormone(FSH),luteinizing hormone(LH),and estradiol(E_(2))were detected.Serum fasting serum lisulin(FINS)and homeostasis model assessment for insulin resistance(HOMA-IR)were measured.Ovulation was observed by HE staining.The protein expressions of p38 MAPK,p-p38 MAPK,ERK1/2 and p-ERK1/2 in ovarian tissues were detected by Western blotting.Results:Compared with the PCOS group,the level of serum TSH,LH,FINS,HOMA-IR,the cystic dilated follicle rate,and ovarian tissue p-p38 MAPK/p38 MAPK,p-ERK1/2/ERK1/2 of the intervention group were decreased,of which the level of FT_(3),FT_(4),T_(3),T_(4),FSH,E_(2) and ovulation rate increased,and the average number of ovulation were increased(P<0.05).Compared with the intervention group,the level of serum TSH,LH,FINS,HOMA-IR,cystic dilated follicle rate

关 键 词：多囊卵巢综合征 菟丝子总黄酮 甲状腺激素 排卵障碍 大鼠 

分 类 号：R285.5[医药卫生—中药学]