雅致小克银汉霉对(+)-诺卡酮的生物转化研究  

Biotransformation of(+)-Nootkatone by Cunninghamella elegans(ATCC9245)

作　　者：王宇彬 李建龙 梁津豪 李恩念 梁微红 徐方方[1,2] 吴云山 黎奔[1] 刘博 蔡桦杨[4] WANG Yubin;LI Jianlong;LIANG Jinhao;LI Ennian;LIANG Weihong;XU Fangfang;WU Yunshan;LI Ben;LIU Bo;CAI Huayang(The Second Clinical Medical College of Guangzhou University of Chinese Medicine,Guangzhou 510006 Guangdong,China;Guangzhou Key Laboratory of Chirality Research on Active Components of Traditional Chinese Medicine,Guangzhou 510006 Guangdong,China;State Key Laboratory of Dampness Syndrome of Chinese Medicine,Guangzhou 510006 Guangdong,China;The Second Affiliatd Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510006,Guangdong,China)

机构地区：[1]广州中医药大学第二临床医学院,广东广州510006 [2]广州市中药活性成分手性研究重点实验室,广东广州510006 [3]省部共建中医湿证国家重点实验室,广东广州510006 [4]广州中医药大学第二附属医院,广东广州510006

出　　处：《中药新药与临床药理》2022年第6期825-829,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省重点领域研发计划(2020B1111110007);广州市中药活性成分手性研究重点实验室(202002010004);省部共建中医湿证国家重点实验室专项(SZ2021ZZ33);广东省自然科学基金项目(2022A1515010103)。

摘　　要：目的探究在雅致小克银汉霉(ATCC 9245)的催化下,(+)-诺卡酮的生物转化反应的效率、产率及产物类型。方法利用雅致小克银汉霉建立生物转化体系,以(+)-诺卡酮为底物进行生物转化,高效液相色谱(HPLC)监测生物转化过程,通过萃取及柱层析的方法分离纯化发酵产物,运用^(1)H-NMR、^(13)C-NMR、HRMS、IR等数据对化合物进行结构确证。结果发酵获得2个主要产物11,12-二羟基诺卡酮(化合物1)和11,12-环氧诺卡酮(化合物2),化合物1含量在发酵第4天达到95.14%的峰值。结论雅致小克银汉霉可以快速、高效催化(+)-诺卡酮转化为其环氧化和羟基化衍生物,并可以高选择性地获得11,12-二羟基诺卡酮。Objective To explore the efficiency,yield and types of products formed by biocatalysis reaction of(+)-nootkatone,which was catalyzed by Cunninghamella elegans.Methods The biotransformation system for(+)-nootkatone(NKT)was established by fungus Cunninghamella elegans.The products were separated by column chromatography and their structures were confirmed by ^(1)H-NMR,^(13)C-NMR,HRMS and IR.The process of biotransformation was monitored and analyzed by HPLC.Results Two main products,11,12-dihydroxylnootkatone(1)and 11,12-epoksynootkatone(2),were obtained by fermentation.The content of compound(1)reached a peak of 95.14%on the 4th day of biotransformation.Conclusion Cunninghamella elegans can quickly and efficiently catalyze the biotransformation of(+)-nootkatone to epoxidized derivatives and hydroxylated derivatives,and to obtain 11,12-dihydroxylnootkatone with high selectivity.

关 键 词：(+)-诺卡酮 雅致小克银汉霉(ATCC 9245) 生物转化 羟基化 环氧化 

分 类 号：R284.1[医药卫生—中药学]