农杆菌介导GUS基因转化籼稻9311的研究  

Agrobacterium-mediated Transformation of the GUS Gene into Indica Rice 9311

作　　者：袁秋林 朱栩婷 丁筠 金宇浩 吕尊富 李飞飞[1] YUAN Qiu-lin;ZHU Xu-ting;DING Yun;JIN Yu-hao;LU Zun-fu;LI Fei-fei(The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province/College of Advanced Agricultural Sciences,Zhejiang A&F University,Lin'an,Zhejiang 311300,China)

机构地区：[1]浙江农林大学现代农学院/浙江省农产品品质改良技术研究重点实验室,浙江临安311300

出　　处：《杂交水稻》2022年第3期20-24,共5页Hybrid Rice

基　　金：2019年浙江农林大学学生科研训练项目(113-2013200134);国家自然科学基金青年基金项目(31401461)。

摘　　要：为建立籼稻品种9311高效稳定的遗传转化体系,本研究以9311的成熟胚为受体材料,利用农杆菌介导法,以潮霉素磷酸转移酶基因作为筛选标记基因,将9311的胚性愈伤组织中β-葡萄糖苷酸酶基因(GUS)导入9311基因组中;通过50mg/L和80mg/L潮霉素对愈伤组织进行2次抗性筛选,经过分化培养后,获得23株再生苗,其中阳性株14株、阳性率60.87%。本研究成功建立了农杆菌介导的9311遗传转化体系,该体系可增加籼稻的阳性植株率,为进一步提高9311等籼稻品种的遗传转化效率提供了技术参考。此外,该方法的改进为籼稻遗传转化和基因功能验证提供了技术基础。In order to establish an efficient and stable genetic transformation system of indica rice variety 9311,its mature embryo was used as the receptor material,and the hygromycin phosphotransferase gene was used as the screening marker by the Agrobacterium-mediated method.Theβ-glucosidase gene(GUS)from the embryogenic calli of 9311 was introduced into the genome of 9311.The calli were screened by hygromycin,and 23 regenerated seedlings were obtained after differentiation culture,of which 14 were positive,with a positive rate of 60.87%.The Agrobacterium-mediated genetic transformation system of 9311 has been successfully established,which can increase the positive plant rate of indica rice,and provides a technical reference for further improving the genetic transformation efficiency of indica rice varieties such as 9311.In addition,the improvement of this method also provides a technical basis for genetic transformation and gene function verification of indica rice.

关 键 词：籼稻 愈伤组织 农杆菌介导 GUS基因 潮霉素 

分 类 号：S511.21[农业科学—作物学] Q75[生物学—分子生物学]