6,6’-Bieckol induces apoptosis and suppresses TGF-β-induced epithelial-mesenchymal transition in non-small lung cancer cells  

在线阅读下载全文

作  者:Yongxin Li Man Liu Kunlong Yang Jun Tian 

机构地区:[1]Department of Biomedicine and Food Science,School of Life Science,Jiangsu Normal University,Xuzhou 221116,China

出  处:《Chinese Herbal Medicines》2022年第2期254-262,共9页中草药(英文版)

基  金:supported by the National Natural Science Foundation of China(81774228,31972171,31671944);Natural Science Foundation by Xuzhou City(KC21160);the PAPD of Jiangsu Higher Education Institutions,and Scientific Research Support Project for Teachers with Doctor’s Degrees(Jiangsu Normal University,17XLR034)。

摘  要:Objective:In this study,the aim was to investigate the inhibitory effect of 6,6’-bieckol on the migration and epithelial-mesenchymal transition(EMT)of non-small cell lung cancer(NSCLC)cells,and explore its potential molecular mechanisms.Methods:Cell migration was measured using a CCK8,wound healing,and transwell migration assay.Apoptosis was determined using an Annexin V/propidium iodide staining.Western blotting and immunofluorescence were used to examine the expression level of apoptosis-related proteins and EMT marker proteins.Results:The results showed that 6,6’-bieckol inhibited migration and induced apoptosis of NSCLC cells.Furthermore,6,6’-bieckol had significantly up-regulated the E-cadherin and down-regulated Snail1 and Twist1 transcriptional levels.6,6’-Bieckol might inhibit TGF-β-induced EMT by down-regulating Snail1 and Twist1 and up-regulating E-cadherin in lung cancer cells.Conclusion:It is suggested that 6,6’-bieckol has the potential to be developed as a therapeutic candidate for lung cancer.

关 键 词:APOPTOSIS 6 6’-bieckol epithelial-mesenchymal transition migration non-small cell lung cancer PHLOROTANNIN traditional Chinese medicine 

分 类 号:R285[医药卫生—中药学]

 

参考文献:

正在载入数据...

 

二级参考文献:

正在载入数据...

 

耦合文献:

正在载入数据...

 

引证文献:

正在载入数据...

 

二级引证文献:

正在载入数据...

 

同被引文献:

正在载入数据...

 

相关期刊文献:

正在载入数据...

相关的主题
相关的作者对象
相关的机构对象