HPLC法测定柔肝降脂胶囊中橙黄决明素、芦荟大黄素、大黄酚和大黄素甲醚  

Determination of aurantio-obtusin,aloe-emodin,chrysophanol and physcion in Rougan Jiangzhi Capsules by HPLC

作　　者：陈海鹰[1] 李莉 薛平 朱珊梅[1] 左小明[1] CHEN Hai-ying;LI Li;XUE Ping;ZHU Shan-mei;ZUO Xiao-ming(Department of Pharmacy,Changzhou Third People's Hospital,Changzhou 213000,China;Changzhou Institute for Food,Drug and Fiber Control,Changzhou 213000,China)

机构地区：[1]常州市第三人民医院药事科,江苏常州213000 [2]常州市食品药品纤维质量监督检验中心,江苏常州213000

出　　处：《现代药物与临床》2022年第5期983-987,共5页Drugs & Clinic

基　　金：常州市第十四批科技计划(应用基础研究指导性)项目(CJ20219027)。

摘　　要：目的建立柔肝降脂胶囊中橙黄决明素、芦荟大黄素、大黄酚和大黄素甲醚的HPLC测定方法。方法采用Waters Symmetry C18色谱柱(250 mm×4.6 mm,5μm);流动相为甲醇–0.1%磷酸水溶液,梯度洗脱;检测波长288 nm;体积流量为1.0 mL/min;柱温30℃;进样量10μL。结果橙黄决明素、芦荟大黄素、大黄酚、大黄素甲醚的线性范围分别为1.59~51.00、0.87~27.92、1.63~52.17、0.22~7.06μg/mL。平均回收率分别为97.55%、100.40%、98.75%、101.30%,RSD值分别为1.93%、1.53%、1.77%、2.23%。4种蒽醌类成分中橙黄决明素和大黄酚的质量分数较高,大黄素甲醚的质量分数较低,不同批次柔肝降脂胶囊样品中芦荟大黄素的质量分数差异较明显。结论所建立的方法可用于柔肝降脂胶囊中4种蒽醌类成分的测定,可为柔肝降脂胶囊的质量控制研究提供参考。Objective To establish HPLC method for determination of aurantio-obtusin,aloe-emodin,chrysophanol,and physcion in Rougan Jiangzhi Capsules.Methods The HPLC analysis was performed on Waters Symmetry C18 column(4.6 mm×250 mm,5μm)with methanol-0.1%phosphoric acid solution by gradient elution.The detection wavelength was set as 288 nm,flow rate was 1.0 mL/min,the column temperature was 30℃,and the sample volume was 10μL.Results The linear ranges of aurantio-obtusin,aloe-emodin,chrysophanol,and physcion were 1.59—51.00,0.87—27.92,1.63—52.17,and 0.22—7.06μg/mL.The recoveries were 97.55%,100.40%,98.75%,and 101.30%with values of 1.93%,1.53%,1.77%,and 2.23%,respectively.Among the four anthraquinones,the contents of aurantio-obtusin and chrysophanol were higher,but the contents of physcion were lower.The contents of aloe-emodin in different batches of Rougan Jiangzhi Capsules samples were significantly different.Conclusion The HPLC method established can determinate the contents of four anthraquinones in Rougan Jiangzhi Capsules,which can provide reference for the quality control research of Rougan Jiangzhi Capsules.

关 键 词：柔肝降脂胶囊 橙黄决明素 芦荟大黄素 大黄酚 大黄素甲醚 高效液相色谱 

分 类 号：R286.02[医药卫生—中药学]