夏枯草茎叶总酚酸提取工艺优化及其化学成分研究  

Optimization of Extraction Technology and Composition Analysis of Total Phenolic Acids from Stems and Leaves of Prunella vulgaris L.

作　　者：罗弘杉 谭知浩 徐春芳 李亚梅[1,2] 谢菁琛 刘月新[1,2] 林丽美 LUO Hongshan;TAN Zhihao;XU Chunfang;LI Yamei;XIE Jingchen;LIU Yuexin;LIN Limei(School of Medicine,Hunan University of Traditional Chinese Medicine,Changsha 410208 Hunan,China;Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province,Changsha 410208 Hunan,China)

机构地区：[1]湖南中医药大学药学院,湖南长沙410208 [2]湘产大宗药材品质评价湖南省重点实验室,湖南长沙410208

出　　处：《中药新药与临床药理》2022年第5期700-706,共7页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：“中医药现代化研究”国家重点研发计划项目(2017YFC1701900);湖南省教育厅重点项目(20A380)。

摘　　要：目的优化夏枯草茎叶总酚酸提取工艺,并分析其化学成分。方法以总酚酸的提取率为指标,在单因素考察的基础上运用Box-Behnken响应面试验设计原理进行3因素3水平的方案设计,得到最佳提取工艺。采用超高效液相色谱-四极杆飞行时间串联质谱(UHPLC-Q-TOF-MS/MS)对夏枯草茎叶总酚酸进行成分分析,通过高效液相色谱(HPLC)定量分析其主要成分咖啡酸和迷迭香酸含量。结果最佳提取工艺条件为:30倍水(V/m)、回流提取120 min、提取3次,总酚酸提取率为4.64%,总酚酸冻干粉得率为15.1%。通过UHPLC-QTOF-MS/MS鉴定出23个化合物,其中酚酸类成分8种。HPLC含量测定结果显示,咖啡酸含量为2.33 mg·g^(-1),迷迭香酸含量为7.37 mg·g^(-1)。结论响应面法优化得到的夏枯草茎叶总酚酸提取工艺简便可行,UHPLC-QTOF-MS/MS和HPLC定性定量分析方法快速灵敏,可为夏枯草茎叶的进一步研究奠定基础。Objective To optimize the extraction process of total phenolic acids from the stems and leaves of Prunella vulgaris and analyze its components.Methods Taking the extraction rate of total phenolic acid from the stems and leaves of P.vulgaris as the index,and based on the results of single factor investigation,the schema design of three factors and three levels was carried out using box-bohnken experimental design principle,and the best extraction process was obtained.Ultra high performance liquid chromatography quadrupole time of flight tandem mass spectrometry(UHPLC-Q-TOF-MS/MS)was used to characterize the components;and the contents of the main components,the caffeic acid and rosmarinic acid,were quantitatively analyzed by liquid chromatography(HPLC).Results The optimum extraction conditions were as follows:30 times water(V/m),and 3 times of extraction process of 120 min reflux.The extraction rate of total phenolic acid was 4.76%.The yield of total phenolic acid extract(freeze-dried powder)was 15.1%.Twenty-three compounds were identified by UHPLC-Q-TOF-MS/MS,including 8 phenolic acids.Content determination by HPLC showed a caffeic acid content of 2.33 mg·g^(-1) and a rosmarinic acid content of 7.37 mg·g^(-1).Conclusion The optimized extraction process of total phenolic acids from the stems and leaves of P.vulgaris is simple and feasible.The UHPLC-Q-TOF-MS/MS and HPLC analysis methods are rapid and sensitive,which can lay a foundation for the further study of the stems and leaves of P.vulgaris.

关 键 词：夏枯草茎叶 总酚酸 提取工艺 响应面法 成分分析 

分 类 号：R284.2[医药卫生—中药学]