抗菌肽重组表达的优化策略  

Strategies for optimization of recombinant expression of antimicrobial peptides

作　　者：宗西翠 张翼(综述) 方彭华 陈玉清(审校)[2] ZONG Xi-cui;ZHANG Yi;FANG Peng-hua;CHEN Yu-qing(不详;Medical Department Experimental Training Center,Hanlin College,Nanjing University of Chinese Medicine,Taizhou 225300,Jiangsu Province,China)

机构地区：[1]南京中医药大学翰林学院医学院实验实训中心,江苏泰州225300 [2]南京师范大学生命科学学院生物化学与生物制品研究所,江苏南京210034 [3]南京中医药大学第一临床医学院,江苏南京210023

出　　处：《中国生物制品学杂志》2022年第4期493-499,507,共8页Chinese Journal of Biologicals

基　　金：国家自然科学基金项目(81573337);江苏省高校自然科学基金面上项目(14KJB310012);江苏高校哲学社会科学研究一般项目(2020SJA2403)。

摘　　要：抗菌肽是由特定基因编码并诱导产生的一类多肽,具有相对分子质量小、热稳定性高、作用机制独特、杀菌范围广等特点,同时在刺激伤口愈合、激素调节及免疫调节等方面有重要作用。基因工程表达技术是大量获得抗菌肽最经济、科学、有效的途径,但由于表达量低、表达产物不稳定、与宿主之间的毒性作用等因素制约了其规模化生产。本文就抗菌肽重组表达过程中的融合标签选择、偏爱密码子选择、最适菌株选择、基因共表达、融合蛋白纯化方法及基因表达设计等方面的优化策略作一综述,以期为抗菌肽的应用和规模化生产提供参考。Antimicrobial peptides are polypeptides with low relative molecular mass,high thermal stability,unique action mechanism and wide bactericidal range,which are encoded by specified genes,and play important roles in stimulating the healing of wound as well as hormone and immune regulations.Gene engineering expression technology is the most economical,scientific and effective way to obtain a large number of antimicrobial peptides.However,antimicrobial peptides are restricted in large-scale production due to their low expression,unstable expressed products and toxic interaction with the host.In this paper,the strategies for optimization of recombinant expression of antimicrobial peptides were briefly reviewed in terms of fusion label selection,preferred codon selection,optimal strain selection,gene coexpression,fusion protein purification method and gene expression design,in order to provide a reference for the application and large-scale production of antimicrobial peptides.

关 键 词：抗菌肽 融合表达 优化策略 

分 类 号：Q78[生物学—分子生物学]