尖孢镰刀菌转化表达松材线虫Bx-apa-2和Bx-apm-2基因dsRNA以及饲喂法介导的RNAi研究  

Double Stranded RNA Expression of Bx-apa-2 and Bx-apm-2 Genes in Fusarium oxysporum Mediated RNAi for Bursaphelenchus xylophilus

作　　者：王猛 宋士林 王旭 田越[1] 张正敏 胡世冉 成新跃 Wang Meng;Song Shilin;Wang Xu;Tian Yue;Zhang Zhengmin;Hu Shiran;Cheng Xinyue(College of Life Sciences,Liaocheng University,Liaocheng Shandong 252059,China;College of Life Science,Beijing Normal University,Beijing 100875,China;Chinese Academy of Inspection and Quarantinc,Beijing 100176,China)

机构地区：[1]聊城大学生命科学学院,山东聊城252059 [2]北京师范大学生命科学学院,北京100875 [3]中国检验检疫科学研究院,北京100176

出　　处：《西南林业大学学报（自然科学）》2022年第3期72-79,共8页Journal of Southwest Forestry University：Natural Sciences

基　　金：国家自然科学基金项目(31700471)资助;山东省自然科学基金项目(ZR2017BC037)资助;北京师范大学生物多样性与生态工程教育部重点实验室开放课题(201702)资助。

摘　　要：通过使用饲喂法介导的RNAi技术,对松材线虫Bx-apa-2和Bx-apm-2基因进行了研究。结果表明:线虫通过取食相应真菌转化体,可以实现持续性的RNA干扰,并造成靶基因的显著性下调。干扰Bx-apa-2基因,线虫主要表现出细小的表型;干扰Bx-apm-2基因,雌虫主要表现出细小的表型,而雄虫则表现出短粗的表型;同时干扰Bx-apa-2和Bx-apm-2基因,线虫主要表现出更为显著的短粗表型。因此,研究结果进一步证明了饲喂法介导的RNAi可以显著性的抑制松材线虫靶基因的表达,并对线虫的表型产生明显的影响。In this study, we studied the effects of Bx-apa-2 and Bx-apm-2 genes by feeding nematodes with the transgenic-expressing-dsRNA fungi. The results showed that the pine wood nematodes could persistently interfered by feeding and led to significantly down-regulate for target genes. By interfering Bx-apa-2gene, nematode showed a relative thinner and smaller phenotype. By interfering the Bx-apm-2 gene, female nematodes were also showed a relative thinner and smaller phenotype, while the males showed a relative shorter and thicker phenotype. In Bx-apa-2 and Bx-apm-2 double-gene interferences group, the phenotype was more significant. In conclusion, for the nematodes, we further proved the interference effects of fungus-mediated RNAi on its gene expression, and observed significantly phenotypes changes as well.

关 键 词：松材线虫 RNA干扰 衔接蛋白2 真菌转化 

分 类 号：S764[农业科学—森林保护学]