Assembly and marker analysis of mitochondrial genomes provide insights into origin,evolution and spread of Brassica juncea(L.)Czern.et Coss.  

作　　者：Liang You Liu Yang Fangying Liu Lei Kang Hao Chen Bin Yang Qian Yang Zhongsong Liu 

机构地区：[1]College of Agronomy,Hunan Agricultural University,Changsha 410128,Hunan,China [2]Guizhou Institute of Oil Crops,Guizhou Academy of Agricultural Sciences,Guiyang 550000,Guizhou,China

出　　处：《The Crop Journal》2022年第3期611-616,共6页作物学报（英文版）

基　　金：China Agriculture Research System(CARS-12);National Natural Science Foundation of China(U20A2029)。

摘　　要：The release of mitochondrial genome sequences provides the basis for characterizing interspecific and intraspecific variation in Brassica mitochondrial genomes.However,few B.juncea(mustard)mitochondrial genomes have been published.We assembled the mitochondrial genomes of three B.juncea subspecies and compared them with previously published genomes.The genomes were phylogenetically classified into A,B,C,and Bna clades.Two variant sites,a transversion(C→A)at nt 79,573 and a 31-bp copy-number variation between nts 65,564 and 65,596,were identified.Based on these variant sites,mitotype-specific sequence markers were developed to characterize the variation among worldwide 558 B.juncea accessions.Three mitochondrial genome types(mitotypes MT1–MT3)were identified.In terms of geographical distribution,MT1 and MT2 accessions were distributed mainly to the north and MT3 to the south of 34°N.Root mustards carried only MT1,leaf and stem mustards carried mainly MT3,and seed mustards carried all three mitotypes,implying that the mitotypes underwent selection during B.juncea domestication.A new form of oil mustard evolved by hybridization between two gene pools in southwest China.

关 键 词：Brassica juncea Mitochondrial genome Mitotypes Molecular markers Migration routes 

分 类 号：S637[农业科学—蔬菜学]