Two types of cinnamoyl-Co A reductase function divergently in accumulation of lignins,flavonoids and glucosinolates and enhance lodging resistance in Brassica napus  

作　　者：Nengwen Yin Bo Li Xue Liu Ying Liang Jianping Lian Yufei Xue Cunmin Qu Kun Lu Lijuan Wei Rui Wang Jiana Li Yourong Chai 

机构地区：[1]Chongqing Engineering Research Center for Rapeseed,Chongqing Key Laboratory of Crop Quality Improvement,College of Agronomy and Biotechnology,Southwest University,Chongqing 400715,China [2]Engineering Research Center of South Upland Agriculture of Ministry of Education,Academy of Agricultural Sciences,Southwest University,Chongqing 400715,China

出　　处：《The Crop Journal》2022年第3期647-660,共14页作物学报（英文版）

基　　金：National Natural Science Foundation of China(31871549,32001579,31830067and 31171177);National Key Research and Development Program of China(2016YFD0100506);Special Financial Aid to Post-doctor Research Fellow of Chongqing(Xm T2018057);“111”Project(B12006);Young Eagles Program of Chongqing Municipal Commission of Education(CY200215)。

摘　　要：Brassica crops,which are of worldwide importance,provide various oil,vegetable and ornamental products,as well as feedstocks for animal husbandry and biofuel industry.Cinnamoyl-Co A reductase(CCR)is the entry point to the lignin pathway and a crucial locus in manipulation of associated traits,but CCRassociated metabolism and traits in Brassica crops have remained largely unstudied except in Arabidopsis thaliana.We report the identification of 16 CCR genes from Brassica napus and its parental species B.rapa and B.oleracea.The Bn CCR1 and Bn CCR2 subfamilies displayed divergent organ-specificity and participation in the yellow-seed trait.Their functions were dissected via overexpression of representative paralogs in B.napus.Bn CCR1 was expressed preferentially in G-and H-lignin biosynthesis and vascular development,while Bn CCR2 was expressed in S-lignin biosynthesis and interfascicular fiber development.Bn CCR1 showed stronger effects on lignification-related development,lodging resistance,phenylpropanoid flux control,and seed coat pigmentation,whereas Bn CCR2 showed a stronger effect on sinapate biosynthesis.Bn CCR1 upregulation delayed bolting and flowering time,while Bn CCR2 upregulation weakened the leaf vascular system in consequence of suppressed G-lignin accumulation.Bn CCR1 and Bn CCR2 were closely but almost oppositely linked with glucosinolate metabolism via inter-pathway crosstalk.We conclude that Bn CCR1 and Bn CCR2 subfamilies offer great but differing potential for manipulating traits associated with phenylpropanoids and glucosinolates.This study reveals the CCR1–CCR2 divergence in Brassicaceae and offers a resource for rapeseed breeding for lodging resistance,yellowseed traits,and glucosinolate traits.

关 键 词：Brassica napus Cinnamoyl-CoA reductase(CCR) Lignin FLAVONOIDS LODGING 

分 类 号：S565.4[农业科学—作物学]