Host-induced gene silencing of multiple pathogenic factors of Sclerotinia sclerotiorum confers resistance to Sclerotinia rot in Brassica napus  

作　　者：Jian Wu Shengliang Yin Li Lin Dongxiao Liu Sichao Ren Wenjing Zhang Wencheng Meng Peipei Chen Qinfu Sun Yujie Fang Cunxu Wei Youping Wang 

机构地区：[1]Key Laboratory of Plant Functional Genomics of the Ministry of Education,Yangzhou University,Yangzhou,China [2]Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding,Yangzhou University,Yangzhou,China

出　　处：《The Crop Journal》2022年第3期661-671,共11页作物学报（英文版）

基　　金：the National Natural Science Foundation of China(32072020,U20A2028,and 31901504);the Jiangsu Agricultural Science and Technology Innovation Fund(CX(20)3120);the Project of Special Funding for Crop Science Discipline Development(yzuxk202006);the Priority Academic Program Development of Jiangsu Higher Education Institutions and the Qinglan Project of Yangzhou University。

摘　　要：Sclerotinia sclerotiorum is generally considered one of the most economically damaging pathogens in oilseed rape(Brassica napus).Breeding for Sclerotinia resistance is challenging,as no immune germplasm available in B.napus.It is desirable to develop new breeding strategies.In the present study,hostinduced gene silencing(HIGS),developed based on RNA interference(RNAi),was applied to protect B.napus from S.sclerotiorum infection.Three pathogenicity genes,the endo-polygalacturonase gene(Ss PG1),cellobiohydrolase gene(Ss CBH),and oxaloacetate acetylhydrolase gene(Ss OAH1),were chosen as HIGS targets.Co-incubation of synthesized double-stranded RNAs(ds RNAs)with S.sclerotiorum in liquid medium significantly reduced the transcript levels of the target genes.Application to plant surfaces of ds RNA targeting the three genes conferred effective protection against S.sclerotiorum.Stable transgenic B.napus plants expressing small interfering RNAs with sequence identity to Ss PG1,Ss CBH,and Ss OAH1 were generated.HIGS transgenic B.napus prevented the expression of S.sclerotiorum target genes,slowed pathogenicity-factor accumulation,impeded fungal growth,and suppressed appressorium formation,thereby conferring resistance to S.sclerotiorum.Simultaneous silencing of Ss PG1,Ss CBH,and Ss OAH1 by stable expression of a chimeric hairpin RNAi construct in B.napus led to enhanced protection phenotypes(with disease lesion size reduced by 36.8%–43.7%).We conclude that HIGS of pathogenic-factor genes of S.sclerotiorum is a promising strategy for controlling Sclerotinia rot in oilseed rape.

关 键 词：Sclerotinia sclerotiorum Brassica napus SIGS HIGS RNAi 

分 类 号：S435.654[农业科学—农业昆虫与害虫防治]