Combined linkage and association mapping reveals two major QTL for stripe rust adult plant resistance in Shaanmai 155 and their haplotype variation in common wheat germplasm  

作　　者：Cai’e Zhou Dan Liu Xu Zhang Qimeng Wu Shengjie Liu Qingdong Zeng Qilin Wang Changfa Wang Chunlian Li Ravi PSingh Sridhar Bhavani Zhensheng Kang Dejun Han Weijun Zheng Jianhui Wu 

机构地区：[1]State Key Laboratory of Crop Stress Biology for Arid Areas,College of Agronomy,Northwest A&F University,Yangling 712100,Shaanxi,China [2]State Key Laboratory of Crop Stress Biology for Arid Areas,College of Plant Protection,Northwest A&F University,Yangling 712100,Shaanxi,China [3]International Maize and Wheat Improvement Center(CIMMYT),El Batan,Texcoco,Estado de Mexico 56237,Mexico

出　　处：《The Crop Journal》2022年第3期783-792,共10页作物学报（英文版）

基　　金：financially the National Natural Science Foundation of China(31871611 and31971890);the National Science Foundation for Young Scientistsin China(31901494 and 31901869);International Cooperation and Exchange of the National Natural Science Foundation of China(31961143019);the Integrated Extension Project of Agricultural Science and Technology Innovation in Shaanxi Province(NYKJ-2021-YL(XN)15)。

摘　　要：The development and deployment of diverse resistance sources in new wheat cultivars underpin the durable control of stripe rust.In the present study,two loci for adult plant resistance(APR),QYr SM155.1 and QYr SM155.2,were identified in the Chinese wheat breeding line Shaanmai 155.QYr SM155.1 was mapped to a 3.0-c M interval between the single-nucleotide polymorphism(SNP)markers AX-109583610 and AX-110907562 on chromosome arm 2 BL.QYr SM155.2 was mapped to a 2.1-c M interval flanked by the SNP markers AX-110378556 and AX-86173526 on chromosome arm 7 AS.A genome-wide association study was used to identify markers associated with APR in a panel of 411 spring wheat lines.Thirteen and 11 SNPs were significantly associated with QYr SM155.1 and QYr SM155.2,respectively,corresponding to physical intervals of 653.75–655.52 Mb on 2 BL and 81.63–83.93 Mb on7 AS.To characterize the haplotype variation and the distribution of these QTL,haplotype analysis was performed based on these SNPs in an independent panel of 1101 worldwide wheat accessions.Three major haplotypes(2 B_h1,2 B_h2,and 2 B_h3)for QYr SM155.1 and four major haplotypes(7 A_h1,7 A_h2,7 A_h3,and 7 A_h4)for QYr SM155.2 were identified.Accessions individually harboring QYr SM155.1_h1 and QYr SM155.2_h1 haplotypes and their combination displayed resistance.Additional assays of 1306 current Chinese cultivars and breeding lines using markers flanking QYr SM155.1 and QYr SM155.2 indicated that the resistance haplotypes of the two QTL were present in respectively 1.45%and 14.16%of lines.Increasing resistance haplotype frequencies at these two loci using marker-assisted selection should benefit wheat production in China.

关 键 词：Linkage and association mapping Stripe rust resistance QYr SM155.1 QYr SM155.2 Haplotype variation 

分 类 号：S512.1[农业科学—作物学]