刚毛柽柳ThZFP3基因的克隆及表达特征分析  

Cloning and expression characteristics of ThZFP3 gene in Tamarix hispida

作　　者：岳远志 贾园园[1] 刘博雅 李垚 周天畅 赵鑫[1] 王超[1] YUE Yuanzhi;JIA Yuanyuan;LIU Boya;LI Yao;ZHOU Tianchang;ZHAO Xin;WANG Chao(State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040,China)

机构地区：[1]东北林业大学林木遗传育种国家重点实验室,哈尔滨150040

出　　处：《植物生理学报》2022年第5期919-928,共10页Plant Physiology Journal

基　　金：国家自然科学基金(31870665);黑龙江省自然科学基金(LH2021C013)。

摘　　要：本研究基于刚毛柽柳转录组数据,克隆获得了一条CCCH型锌指蛋白的全长基因,并命名为ThZFP3。ThZFP3基因开放阅读框(ORF)全长1335 bp,编码444个氨基酸,编码蛋白的分子质量为48.49kDa,理论等电点为8.08。进化树分析结果表明,ThZFP3与川桑和毛果杨ZFP的亲缘关系最近。亚细胞定位结果显示ThZFP3定位于细胞核中。在酵母中,全长ThZFP3没有转录激活活性,但在其羧基端(C端)具有转录激活活性,转录激活域位于C端的56个氨基酸区段。此外,实时荧光定量PCR分析结果显示,ThZFP3的表达受NaCl和PEG的诱导,表明ThZFP3可能参与了刚毛柽柳对盐和干旱胁迫的应答反应。以上研究结果为进一步研究ThZFP3在刚毛柽柳耐盐抗旱中的功能及作用机制提供了依据。In this study,a CCCH-type zinc finger protein gene named as Th ZFP3 was isolated from the transcriptome cDNA library of Tamarix hispida.The opening reading frame(ORF)length of ThZFP3 was 1335bp,encoding 444 amino acids.The molecular weight of ThZFP3 protein was 48.49 kDa,with isoelectric point 8.08.Phylogenetic tree analysis showed that ThZFP3 was closely related to ZFP of Morus notabilis and Populus trichocarpa.Subcellular localization analysis showed that the ThZFP3 was specifically located in the cell nucleus.The yeast transcriptional activation experimental analysis indicated that though the full-length ThZFP3 did not have transcriptional activation activity,the carboxy-terminal(C-terminal)region of ThZFP3 had transcriptional activation activity in yeast,and the transcriptional activation region was located in 56 amino acids at the C-terminal region.Furthermore,quantitative real-time PCR assay revealed that the expression of ThZFP3 gene was significantly up-regulated under NaCl and PEG treatments,suggesting that Th ZFP3 may be involved in the response of T.hispida to salt and drought stress.These results provide a theoretical basis for further analyzing the function and regulation mechanism of ThZFP3 involved in stress response of T.hispida.

关 键 词：刚毛柽柳 CCCH锌指蛋白 非生物胁迫 转录激活 表达分析 

分 类 号：S793.5[农业科学—林木遗传育种]