Bombyx mori U-shaped regulates the melanization cascade and immune response via binding with the Lozenge protein  

作　　者：Kui Zhang Juan Tan Xiangwei Hao Houyi Tang Muhammad Nadeem Abbas Jingjing Su Yongyue Su Hongjuan Cui 

机构地区：[1]State Key Laboratory of Silkworm Genome Biology,College of Sericulture,Textile and Biomass Sciences,Southwest University,Chongqing,400716,China [2]Chongqing Reproductive and Genetics Institute,Chongqing Obstetrics and Gynecology Hospital,Chongqing,400013,China [3]Department of Orthopaedic,920th Hospital of Joint Logistics Support Force of Chinese People’s Liberation Army,Kunming,650032,China [4]Cancer Center,Reproductive Medicine Center,Medical Research Institute,Southwest University,Chongqing,400716,China

出　　处：《Insect Science》2022年第3期704-716,共13页昆虫科学（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(31802142);the Doctorial Start-up Fund of Southwest University(SWU120019,020023);the Fundamental Research Funds for the Central Universities(XDJK2019C089);the China Postdoctoral Science Foundation(2019T120801 and 2017M620408);the Opening Foundation of State Key Laboratory of Silkworm Genome Biology(SKLSGB-ORP202003).

摘　　要：Zinc finger protein,an important transcription factor,regulates gene expression associated with various physiological and pathological processes.U-shaped,belong to the Friend of GATA(FOG)transcription factor,plays a crucial role in hematopoiesis by interacting with the GATA transcription factor as a co-factor.However,little is known about its functions in insects.In the present study,a U-shaped cDNA was identified and characterized from the silkworm Bombyx mori and its potential roles in innate immunity investigated.The predicted silkworm U-shaped amino acid sequence contained a classical nuclear localization signal(NLS)motif“GESSPKRRRR”at position 45CU459,and arginine residues at position 456 and 478 are the critical sites of the NLS.U-shaped mRNA was detected in all tested tissues of the B.mori;however,the highest levels were found in the hemocytes.U-shaped mRNA expression levels were upregulated in the hemocyte after the Escherichia coli and Staphylococcus aureus challenge.Furthermore,U-shaped knockdown significantly reduced the melanization process and suppressed the expression of melanization-associated genes,including PPO1,PPO2,PPAE and BAEE.In addition,U-shaped interacts with Lozenge protein to regulate the innate immune response of the insect.Our results revealed that U-shaped binds directly to Lozenge protein to modulate the melanization process and innate immune responses in silkworm.

关 键 词：Bombyx mori LOZENGE melanization response SILKWORM U-SHAPED 

分 类 号：Q96[生物学—昆虫学]