基于线粒体16S rRNA和COI序列的江苏启东海域4种贝类遗传多样性分析  

Genetic diversity of four shellfishes in sea area of Qidong,Jiangsu Province based on mitochondrial 16S rRNA and COⅠsequences

作　　者：闫永斌 葛玉双 程起群[1] 范瑞良 李楠楠 全为民[1] YAN Yongbin;GE Yushuang;CHENG Qiqun;FAN Ruiliang;LI Nannan;QUAN Weimin(East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China;College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, China)

机构地区：[1]中国水产科学研究院东海水产研究所,上海200090 [2]上海海洋大学海洋科学学院,上海201306

出　　处：《海洋渔业》2022年第2期201-208,共8页Marine Fisheries

基　　金：中央级公益性科研院所基本科研业务费(2015M01)。

摘　　要：采集启东海域的4种贝类,即日本镜蛤(Dosinia japonica)、四角蛤蜊(Mactra veneriformis)、西施舌(Coelomactra antiquata)和中国蛤蜊(Mactra chinensis),对其线粒体16S rRNA和COI基因片段进行PCR扩增和测序,并进行遗传多样性分析。结果显示,西施舌和四角蛤蜊的遗传多样性处于一个相对较高的水平,而日本镜蛤和中国蛤蜊的遗传多样性水平相对较低,且同一物种COI基因的变异程度一般高于16S rRNA基因。研究结果可为江苏启东4种贝类种质资源的管理、保护和监测提供参考。In order to investigate the genetic diversity background of shellfishes in sea area of Qidong City, Jiangsu Province, we set twenty seven sampling sites and collected 2 031 individuals of four kinds of shellfishes, Dosinia japonica, Mactra veneriformis, Coelomactra antiquate, and Mactra chinensis along the coast of Qidong. Among these samples, the genomic DNA of 120 individuals, 30 individuals of each species, were abstracted following the instruction of kit, and the mitochondrial 16 S ribosomal RNA(16 S rRNA) and cytochrome oxidase subunit I(COⅠ) gene sequences were PCR amplified, sequenced, and analyzed. Results showed that the base composition structure of 16 S rRNA and COI gene fragments of these four kinds of shellfishes demonstrated significant higher A+T(%) than C+G(%), and the proportion of base C was the lowest. The proportion of A to T in 16 S rRNA gene sequent was a little different, and the proportion of base T in the COⅠ gene sequence was significantly higher than the proportion of base A.In addition, the haplotype diversity indexes of 16 S rRNA and COⅠ gene markers of Dosinia japonica were 0.889 and 0.984, and the nucleotide diversity indexes were 0.006 5 and 0.004 24, respectively;the haplotype diversity indexes of the two gene markers of Mactra veneriformis were respectively 0.933 and 1, the nucleotide diversity indexes were respectively 0.00682 and 0.0088;the haplotype diversity indexes of the two gene markers of Coelomactra antiquata were 0.869 and 0.961, and the nucleotide diversity indexes were 0.009 37 and 0.013 47, respectively;the haplotype diversity indexes of the two gene markers of Mactra chinensis were 0.411 and 0.874, and the nucleotide diversity indexes were 0.001 74 and 0.003 49, respectively. Overall, the genetic diversities of C. antiquata and M. veneriformis were at a relatively high level, while the genetic diversities of D. japonica and M. chinensis were at a relatively low level. As far as the same species was concerned, the variation degree of COⅠ gene was generally hi

关 键 词：日本镜蛤 四角蛤蜊 西施舌 中国蛤蜊 遗传多样性 16S rRNA COI 

分 类 号：S917.4[农业科学—水产科学]