不同盐度水平对金钱鱼幼鱼鳃线粒体丰富细胞结构的影响  

Effects of different salinities on the structure of gill mitochondria-rich cells of juvenile Scatophagus argus

作　　者：刘鉴毅[1,2,3] 余焱方 冯广朋 赵峰[1,2,3] 孙雪娜 王妤[1,3] 邹雄 黄晓荣 李琪[1,2] 孙艳秋 LIU Jianyi;YU Yanfang;FENG Guangpeng;ZHAO Feng;SUN Xuena;WANG Yu;ZOU Xiong;HUANG Xiaorong;LI Qi;SUN Yanqiu(East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China;National Demonstration Center for Expermental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;Key Scientific Observing and Experimental Station of Fisheries Resource and Environment in the Yangtze Estuary and East China Sea, Ministry of Agriculture and Rural Affairs, Shanghai 200090, China)

机构地区：[1]中国水产科学研究院东海水产研究所,上海200090 [2]水产科学国家级实验教学示范中心,上海海洋大学,上海201306 [3]农业农村部东海与长江口渔业资源环境科学观测实验站,上海200090

出　　处：《海洋渔业》2022年第2期209-218,共10页Marine Fisheries

基　　金：中央基本科研业务费重点项目计划(2019Z01);国家重点研发计划(2019YFD 0900405)。

摘　　要：为研究盐度对金钱鱼(Scatophagus argus)幼鱼渗透调节的影响,将(6.31±0.25)g金钱鱼置于不同盐度(0、5、10、20、30、35)下养殖30 d,观察分析不同盐度下金钱鱼幼鱼鳃线粒体丰富细胞(mitochondria-rich cells,MRCs)形态结构的变化。结果显示:盐度5组次级鳃小片直径(11.750±1.388)μm,盐度10组初级鳃小片直径(92.050±9.408)μm,盐度35组初级鳃小片直径(80.250±4.682)μm,与对照组相比差异显著(P<0.05)。MRCs顶膜开口分为:突起型(Ⅰ型)、凹陷型(Ⅱ型)、深洞型(Ⅲ型),以及一种Ⅰ型的过渡类型。研究表明,金钱鱼幼鱼在不同盐度条件下MRCs均发生适应性的结构改变,有利于金钱鱼在不同盐度水体中生存,这种适应性结构变化与金钱鱼具有较强的盐度适应性密切相关。Scatophagus Argus,also known as golden drum fish,belongs to Perciformes and is widely distributed in the Indo-Pacific waters.It is a widely salty subtropical bony fish and has strong adaptability in osmotic regulation.The change of water environment salinity will destroy the homeostasis of fish’s internal environment.Under the seawater environment with different salinities,gill mitochondria rich cells mainly play the role of ion absorption and ion secretion,secreting chloride ions(Cl^(-))to the outside under the condition of low permeability,and secreting and absorbing sodium ions(Na^(+))and potassium ions(K^(+))under the condition of high permeability.Its quantity and shape will change accordingly due to the change of external salinity,so as to adapt to the change of external water environment salinity and play an important role in fish osmotic pressure regulation.The experiment set the salinity gradient as 0,5,10,20,30 and 35.Taking the local natural seawater(salinity 20)as the control group,salinity 5,10,30 and 35 were prepared by adding different proportions of sea crystal to fresh water.Three juveniles were randomly selected from each water tank,dissected on the ice plate,and the gill filaments of the second gill arch on the left were collected.After calibrating the photos taken by the optical microscope with Imager proplus 6.0 software,the diameter of primary gill flakes and secondary gill flakes on the gill filaments of different groups of goldfish was measured,six groups of data at the same part were randomly measured,and then the average value was taken.The experimental data(SD±SEM)were recorded in SPSS 24.One way analysis of variance(ANOVA)was performed on the software,and Duncan multiple comparison method was used to test the difference between groups(P=0.05).The results were tabulated in Excel 2016 for analysis.Results showed that the mitochondria of juvenile Scatophagus argus were abundant in freshwater environment,and the diameter of primary gill flakes was(57.350±9.114)μm,significantly lower t

关 键 词：金钱鱼幼鱼 盐度 显微镜技术 线粒体丰富细胞 

分 类 号：S917.4[农业科学—水产科学]