食用黄花菜SSR标记开发及指纹图谱库构建  

The development of SSR markers and construction of fingerprint library for edible daylily

作　　者：刘新星[1] 欧巧明[1] 罗俊杰[1] 李忠旺[1] 石有太[1] 崔文娟[1] LIU Xinxing;OU Qiaoming;LUO Junjie;LI Zhongwang;SHI Youtai;CUI Wenjuan(Biotechnology Institute,Gansu Academy of Agricultural Sciences,Lanzhou 730070,China)

机构地区：[1]甘肃省农业科学院生物技术研究所,甘肃兰州730070

出　　处：《食品与发酵工业》2022年第12期188-195,共8页Food and Fermentation Industries

基　　金：甘肃省农业科学院中青年基金项目(2019GAAS39;2019GAAS43);甘肃省农业科学院科技成果转化项目(2019GAAS-CGZH03)。

摘　　要：利用转录组测序技术开发SSR标记,旨在为食用黄花菜种质资源鉴定、评价及遗传多样性分析提供技术支撑。测序共得到104177个Unigene,总长度为79106833 bp,平均长度为759 bp,利用MISA软件对Unigene进行搜索,共检测到15890个SSR,其中单核苷酸、二核苷酸和三核苷酸重复占比较高,分别为18.57%、27.23%和44.26%。设计合成SSR引物114对,通过聚丙烯酰氨凝胶电泳最终筛选出20对核心引物,20对引物在43份黄花菜种质中共检测到88个等位变异,每个位点的等位变异数为3~8个,平均为4.4个,多态信息含量(polymorphism information content,PIC)值变幅为0.23~0.77,平均为0.4995。依据扩增产物的片段大小确定了每个SSR位点的等位变异以及对应的参照品种,建立了基于SSR的DNA指纹鉴定技术体系,并构建了43份黄花菜种质的DNA指纹数据。该研究开发的SSR标记具有丰富的多态性。The SSR markers were developed by transcriptome sequencing technology to provide technical support for the identification,evaluation and genetic diversity analysis of edible daylily germplasm resources.The results showed that a total of 104177 unigenes containing 79106833 bpin sequence,with an average length of 759 bp was obtained.The Unigene was searched using MISA software,and a total of 15890 SSRs were detected which including single nucleotides,dinucleotides and trinucleotides accounting for a relatively high proportion of 18.57%,27.23%and 44.26%,respectively.A total of 114 pairs of SSR primers were used for the screening,and finally,20 pairs of primers were screened out by polyacrylamide gel electrophoresis.A total of 88 allelic variants were detected in 43 daylily germplasms,and the number of allelic variants at each locus was 3-8.The polymorphism information content(PIC)was ranged from 0.23 to 0.77.Based on the fragment size of the amplified product,the allelic variation of each SSR locus and the corresponding reference variety were determined,and a DNA fingerprint identification technology system was constructed.The SSR markers developed in this study are rich in polymorphism.

关 键 词：黄花菜 SSR标记 指纹图谱 

分 类 号：S644.3[农业科学—蔬菜学]