高致病性FAdV-4分离株fiber2结构蛋白表达和细胞内定位的分析  

Analysis of the expression and intracellular localization of the structural protein fiber2 in a highly pathogenic FAdV-4 strain

作　　者：李梅珍 洪琴 邓翔飞 陈芳芳[1] LI Meizhen;HONG Qin;DENG Xiangfei;CHEN Fangfang(School of Animal Science and Technology,Anhui Agricultural University,Hefei 230036)

机构地区：[1]安徽农业大学动物科技学院,合肥230036

出　　处：《安徽农业大学学报》2022年第2期259-264,共6页Journal of Anhui Agricultural University

基　　金：安徽省教育厅重点项目(KJ2020A0124)资助。

摘　　要：初步鉴定一株高致病性4型禽腺病毒(fowl adenovirus serotype 4, FAdV-4)并研究其生物学特性。应用PCR、动物实验、免疫印迹技术和激光共聚焦鉴定其致病性和传播途径,分析fiber2蛋白的表达特性及其在细胞内定位。结果显示,所分离的毒株为FAdV-4型强毒株,对鸡的致死率达100%,对鸭无明显致病性;病毒可以经过滴鼻点眼感染而非经过口腔感染;PCR扩增的fiber2基因的大小为1 440 bp,所构建的含fiber2基因的重组真核和原核表达质粒均可正确表达;共聚焦结果显示,fiber2蛋白主要定位于细胞核。结果可为进一步研究安徽省地区高致病性禽腺病毒的感染和疫苗制备提供基础。A highly pathogenic fowl adenovirus type 4(FAdV-4) was identified and its biological characteristics were studied. PCR, animal experiment, Western Blot and laser confocal microscope were used to identify its pathogenicity and transmission routes, and to analyze the expression characteristics and intracellular localization of fiber2 protein. The results showed that the isolated strain was a highly pathogenic strain of FAdV-4, with a 100% mortality rate in chickens and no significant pathogenicity in ducks. The strain could be infected by nose and eye dropping rather than through the oral cavit. The fiber2 gene amplified by PCR was 1 440 bp in length.The recombinant eukaryotic and prokaryotic expression plasmids containing the fiber2 gene were successfully constructed, and both could correctly express the protein in the cells. The laser confocal microscope observation showed that the fiber2 protein was mainly located in the nucleus. The result provides a basis for the further study on the infection of highly pathogenic FAdV and the vaccine preparation in Anhui Province.

关 键 词：FAdV-4 分离鉴定 fiber2 蛋白表达 细胞定位 

分 类 号：S852.657[农业科学—基础兽医学]