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作 者:周圣博 张芷赫 王惠 苗青 徐树军 关萍[1,2] 史静耸 ZHOU Sheng-Bo;ZHANG Zhi-He;WANG Hui;MIAO Qing;XU Shu-Jun;GUAN Ping;SHI Jing-Song(College of Bioscience and Technology,Shenyang Agricultural University,Key Lab of Global Changes and Biological Invasions,Liaoning Province,Shenyang 110866;Liaoning Panjin Wetland Ecosystem National Observation and Research Station,Panjin 124112;College of Life Science,Liaoning Normal University,Dalian 116081;Liaoning Shuangtai Estuary Wetland Ecosystem Research Station,Panjin 124112;State-owned Asset Management Office of Shenyang Agricultural University,Shenyang 110866;Key Laboratory of Vertebrate Evolution and Human Origins of Chinese Academy of Sciences,Institute of Vertebrate Paleontology and Paleoanthropology,Chinese Academy of Sciences,Beijing 100044,China)
机构地区:[1]沈阳农业大学生物科学技术学院,辽宁省全球变化与生物入侵重点实验室,沈阳110866 [2]辽宁盘锦湿地生态系统国家野外科学观测研究站,盘锦124112 [3]辽宁师范大学生命科学学院,大连116081 [4]辽宁双台河口湿地生态系统国家定位观测研究站,盘锦124112 [5]沈阳农业大学国有资产管理处,沈阳110866 [6]中国科学院脊椎动物演化与人类起源重点实验室,中国科学院古脊椎动物与古人类研究所,北京100044
出 处:《动物学杂志》2022年第3期440-446,共7页Chinese Journal of Zoology
基 金:2021年辽宁省普通本科高等学校校际合作项目[资源共享(2021-57)];2021年沈阳农业大学优秀本科毕业论文(设计)培育计划项目(No.48)。
摘 要:2021年9月在沈阳市蒲河湿地公园(41°41′54″N,122°52′19″E,海拔35 m)采集到3号无尾两栖类动物,经形态特征比较确认为侧褶蛙属(Pelophylax)金线侧褶蛙种组(P.plancyi species complex)的物种。基于线粒体12S rRNA和16S rRNA基因联合构建的侧褶蛙属部分物种最大似然系统发育树显示,采集到的侧褶蛙标本与朝鲜侧褶蛙(P.chosenicus)聚为一支,且具有较高的支持率(0.95)。综合形态和系统发育比较,确定采集到的标本为无尾目(Anura)蛙科(Ranidae)侧褶蛙属的朝鲜侧褶蛙,系中国两栖动物分布新记录种。Three tailless amphibian specimens were collected in Puhe Wetland Park(41°41′54″N,122°52′19″E,elevation 35 m)in Shenyang in September 2021(Fig.1,with Fig.3 showing the habitat).In this study,the morphological characteristics of the collected specimens were compared and analyzed in detail with P.chosenicus and P.plancyi.The 12S rRNA and 16S rRNA gene of 3 samples were amplified and sequenced by second-generation high-throughput sequencing.The 12S rRNA and 16S rRNA gene information of 19 species of Pelophylax distributed in China and abroad(including a species of Rana as an outgroup)were obtained in NCBI GenBank database and existing reports.Phyml 3.0 to construct maximum likelihood phylogenetic tree.Morphological analysis shows that there are interspecific differences between our specimens and P.plancyi,which is consistent with the type origin species of P.chosenicus.The results of ML phylogenetic tree show that the Pelophylax specimens collected in Shenyang Puhe Wetland Park and the type origin(Korea)specimens of P.chosenicus gather into one branch(Fig.2),with a high support rate(95%).By comprehensive comparison with morphological and phylogenetic analysis,the specimens we collected should be the P.chosenicus of the genus Pelophylax in the family Anura,and be a new record of amphibian distribution in China.
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