SARS-CoV-2-specific antibody response characteristics in COVID-19 patients of different ages  

作　　者：Linfang Lu Siqi Yu Min Liu Yang Li Qing Lei Mingxi Lin Danyun Lai Shujuan Guo Hewei Jiang Hongyan Hou Yunxiao Zheng Xuening Wang Mingliang Ma Bo Zhang Hong Chen Junbiao Xue Hainan Zhang Huan Qi Ziyong Sun Feng Wang Xionglin Fan Zhaowei Xu 

机构地区：[1]Key Laboratory of Gastrointestinal Cancer(Fujian Medical University),Ministry of Education,Fuzhou 350122,China [2]Laboratory of Scientific Research,School of Basic Medical Sciences,Fujian Medical University,Fuzhou 350122,China [3]Fujian Key Laboratory of Tumor Microbiology,Department of Medical Microbiology,Fujian Medical University,Fuzhou 350122,China [4]Shanghai Center for Systems Biomedicine,Key Laboratory of Systems Biomedicine(Ministry of Education),Shanghai Jiao Tong University,Shanghai 200240,China [5]Department of Pathogen Biology,School of Basic Medicine,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430074,China [6]Department of Clinical Laboratory,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430074,China

出　　处：《Acta Biochimica et Biophysica Sinica》2022年第4期556-564,共9页生物化学与生物物理学报（英文版）

基　　金：supported by the grant from the National Natural Science Foundation of China(No.32000027)。

摘　　要：Age has been found to be one of the main risk factors for the severity and outcome of COVID-19.However,differences in SARS-CoV-2 specific antibody responses among COVID-19 patients of different age groups remain largely unknown.In this study,we analyzed the IgG/IgM responses to 21 SARS-CoV-2 proteins and 197 peptides that fully cover the spike protein against 731 sera collected from 731 COVID-19 patients aged from 1 to 92 years.We show that there is no overall difference in SARS-CoV-2 antibody responses in COVID-19 patients in the 4 age groups.By antibody response landscape maps,we find that the IgG response profiles of SARS-CoV-2 proteins are positively correlated with age.The S protein linear epitope map shows that the immunogenicity of the S-protein peptides is related to peptide sequence,disease severity and age of the COVID-19 patients.Furthermore,the enrichment analysis indicates that low S1 IgG responses are enriched in patients aged<50 and high S1 IgG responses are enriched in mild COVID-19 patients aged>60.In addition,high responses of non-structural/accessory proteins are enriched in severe COVID-19 patients aged>70.These results suggest the distinct immune response of IgG/IgM to each SARS-CoV-2 protein in patients of different age,which may facilitate a deeper understanding of the immune responses in COVID-19 patients.

关 键 词：SARS-CoV-2 protein microarray age humoral immunity IgG and IgM non-structural/accessory protein 

分 类 号：R563.1[医药卫生—呼吸系统]