小鼠脊髓损伤后Bmi-1在脊髓中的表达变化  

Expression of Bmi-1 in spinal cord after mice spinal cord injury

作　　者：金瑶瑶 赵伟华[3] 高钰 肖晨宇 张永杰[1,2] JIN Yaoyao;ZHAO Weihua;GAO Yu;XIAO Chenyu;ZHANG Yongjie(Department of Human Anatomy,Nanjing Medical University,Nanjing 211166;Key Laboratory for Aging&Diseases,Nanjing Medical University,Nanjing 211166;Department of Orthopaedics,the Affiliated Changzhou Hospital of Traditional Chinese Medicine,Nanjing University of Chinese Medicine,Changzhou 213003;the First Clinical Medical College,Nanjing Medical University,Nanjing 211166,China)

机构地区：[1]南京医科大学人体解剖学系,江苏南京211166 [2]南京医科大学衰老及相关疾病研究重点实验室,江苏南京211166 [3]南京中医药大学附属常州市中医医院骨科,江苏常州213003 [4]南京医科大学第一临床医学院,江苏南京211166

出　　处：《南京医科大学学报（自然科学版）》2022年第6期780-789,共10页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81472081);2020年江苏省青蓝工程资助项目。

摘　　要：目的:检测小鼠脊髓损伤(spinal cord injury,SCI)后B淋巴瘤Mo-MLV插入区1(B cell-specific MLV integration site-1,Bmi-1)在脊髓中的表达变化。方法:取2月龄C57Bl/6小鼠,利用LISA脊髓损伤造模仪制作小鼠第9胸椎节段(T9)中度脊髓钝挫伤模型,通过免疫荧光染色与Western blot检测,初步观察SCI后1、3、7、14、28 d时Bmi-1在脊髓中的表达变化与细胞定位。结果:Bmi-1在脊髓中的表达,于损伤后1 d即上调并达峰值,至损伤后28 d仍保持较高水平,其表达变化与增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)的表达变化趋势一致。免疫荧光染色结果显示:SCI后,Bmi-1与离子钙接头蛋白分子1(ionized calcium binding adapter molecule 1,Iba-1)、髓鞘碱性蛋白(myelin basic protein,MBP)、神经元核心抗原(neuronal nuclei antigen,NeuN)及血小板内皮细胞黏附分子1(platelet and endothelial cell adhesion molecule-1,PECAM-1/CD31)共表达;Bmi-1与神经胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)无共表达。结论:小鼠脊髓损伤后脊髓中Bmi-1表达上调,主要定位于小胶质细胞、髓鞘细胞、神经元与血管内皮细胞,且在小胶质细胞中的表达尤为显著。提示Bmi-1在脊髓损伤后的表达变化可能与小胶质细胞的增殖与活化、内源性髓鞘再生及血管内皮细胞的活化有关,可能在脊髓损伤后的病理过程中具有重要意义。Objective:This study aims to explore the expression of B cell-specific MLV integration site-1(Bmi-1)in mice spinal cord after spinal cord injury(SCI).Methods:Two month-old C57Bl/6 mice received moderate contusion SCI at T9 using LISA impactor.The expression and cellular localization of Bmi-1 in spinal cord at the day of 1,3,7,14 and 28 post SCI were detected by Western blot and immunofluorescence staining.Results:The results of Western blot showed that the expression of Bmi-1 was increased and achieved a peak value at day one post SCI,and which was higher than normal till to the day 28 post SCI.The tendency of the Bmi-1 expression was consistent with the expression of proliferating cell nuclear antigen(PCNA).The results of the immunofluorescence staining showed that the Bmi-1 was increased and co-localized with ionized calcium binding adapter molecule 1(Iba-1),myelin basic protein(MBP),neuronal nuclei antigen(NeuN),and platelet and endothelial cell adhesion molecule-1(PECAM-1/yCD31),but which was not co-localized with glial fibrillary acidic protein(GFAP)after SCI.Conclusion:The expression of Bmi-1 was increased and mainly localized in microglia,myelin cells,neuron and endothelium,and especially in microglia after SCI.It suggests that the expression of Bmi-1 may relate with the proliferation and activation of microglia,the endogenous remyelination,and the activity of the endothelium.The Bmi-1 may involve in the pathological process after SCI.

关 键 词：脊髓损伤 BMI-1 细胞定位 小鼠 

分 类 号：R651.2[医药卫生—外科学]