香连外洗液对氟康唑抗耐药白念珠菌增效作用的转录组学研究  

Research on Synergism of Xianglian Lotion to Fluconazole against Drug-resistance Candida albicans Based on RNA-seq Technology

作　　者：王平[1] 范瑞强[2] 柏彩英[3] WANG Ping;FAN Ruiqiang;BAI Caiying(School of Medicine,Datong Universality,Shanxi Province,Datong 037009,China;Department of Dermatology,Guangdong Hospital of Traditional Chinese Medicine,Guangdong Province,Guangzhou 510120,China;Inspection Department,Maternity and Childcare Centers of Guangdong Province,Guangdong Province,Guangzhou 510020,China)

机构地区：[1]山西大同大学医学院,山西大同037009 [2]广东省中医院皮肤科,广东广州510120 [3]广东省妇幼保健院检验科,广东广州510020

出　　处：《光明中医》2022年第9期1581-1584,共4页GUANGMING JOURNAL OF CHINESE MEDICINE

基　　金：山西省应用基础研究计划(No.201701D221243)。

摘　　要：目的对2种不同处理后的白念珠菌ATCC10231的差异基因进行检测,并对其差异基因进行转录组学研究,明确香连洗液对氟康唑抗耐药白念珠菌的增效机制。方法采用转录组测序的方法,对不同处理后的白念珠菌ATCC1023的差异基因进行测定,并对差异表达基因进行GO分析和KEGG分析。结果香连外洗液+氟康唑作用后与氟康唑单独作用后相比较,ATCC10231有547个基因FPKM值上调,963个基因FPKM值下调。GO分析显示,这些差异基因在BP分类中,主要涉及代谢过程、合成过程、氧化还原过程、转运;这些基因的细胞组分主要富集在细胞膜、膜组件等;分子功能主要富集在转运活动、结合活动等。KEGG分析显示,2种不同处理后,ATCC10231差异基因主要富集在耐药相关的MFS转运蛋白家族以及代谢途径。结论经香连外洗液作用后,ATCC10231细胞内代谢活动改变、细胞膜成分发生变化;香连外洗液降低了ATCC10231耐药基因的表达,增强了菌株对氟康唑的敏感性,影响了某些代谢途径。Objective In order to clear and definite synergistic mechanism of Xianglian lotion to fluconazole to drug-resistance Candida albicans,transcriptome was sequenced by the RNA-seq.Methods Differential expressed gene of ATCC10231 was identified by the RNAseq,and was analyzed by GO analysis and KEGG pathway analysis.Results 547 genes FPKM index up regulated,963 genes FPKM index down regulated.Differential expressed genes were enriched in metabolic process,biosynthetic process,oxidation-reduction process,transport and metabolic pathway by KEGG pathway analysis.Conclusions Xianglian lotion reduced expression of drug-resistant genes and changed some metabolic pathway.

关 键 词：氟康唑 香连外洗液 白念珠菌 RNA-seq技术 实验研究 

分 类 号：R285[医药卫生—中药学]