洋金花叶化学成分及其抗炎活性研究  

Chemical constituents from the leaves of Datura metel and their anti-inflammatory activities

作　　者：刘艳[1] 荣晓惠 谭金燕 潘娟[1] 管伟[1] 匡海学[1] 杨炳友[1] LIU Yan;RONG Xiao-hui;TAN Jin-yan;PAN Juan;GUAN Wei;KUANG Hai-xue;YANG Bing-you(Ministry of Education Key Laboratory for Basic and Applied Research of Northern Medicines,Heilongjiang University of Chinese Medicine,Harbin 150040,China;Shanxi University of Chinese Medicine,Jinzhong 030619,China)

机构地区：[1]黑龙江中医药大学,教育部北药基础与应用研究重点实验室,黑龙江哈尔滨150040 [2]山西中医药大学,山西晋中030619

出　　处：《中成药》2022年第6期1829-1839,共11页Chinese Traditional Patent Medicine

基　　金：国家重点研发计划(2018YFC1707100,2018YFC1707103);国家自然科学基金(81773883,81903781);博士后科学基金(2018M631978);黑龙江省自然科学基金优秀青年项目(YQ2019H029);黑龙江中医药大学校基金(2018pt01,2018bs03);黑龙江省“尖雁”团队(2019-2023)。

摘　　要：目的 研究洋金花Datura metel L.叶化学成分及其抗炎活性。方法 采用大孔吸附树脂、硅胶、ODS、HPLC法对洋金花叶70%乙醇提取物进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构,采用RAW264.7模型评价其体外抗炎活性。结果 从中分离得到28个化合物,分别鉴定为7,27-dihydroxy-1-oxowitha-2,5,24-trienolide(1)、acnistoferin(2)、baimantuoluoline K(3)、daturafoliside G(4)、daturafoliside H(5)、daturafoliside I(6)、daturafoliside R(7)、daturafoliside S(8)、daturametelin A(9)、daturametelin I(10)、daturametelin J(11)、daturataturin A(12)、baimantuoluoside H(13)、(22R)-27-hydroxy-7α-methoxy-1-oxowitha-3,5,24-trienolide(14)、柯里拉京(15)、desmethylagrimonolide 6-O-β-D-glucopyranoside(16)、仙鹤草内酯-6-O-β-D-吡喃葡萄糖苷(17)、丁香脂素(18)、丁香脂素-4-O-β-D-吡喃葡萄糖苷(19)、1,6-di-O-coumaroyl glucopyranoside(20)、滨蒿内酯(21)、东莨菪内酯(22)、野蔷薇亭(23)、(-)-loliolide(24)、(+)-isololiolide(25)、邻苯二甲酸二丁酯(26)、对羟基苯甲醛(27)、没食子酸乙酯(28)。化合物9、20能抑制LPS诱导细胞释放NO,IC_(50)分别为25.14、16.26μmol/L。结论 化合物15~17、20、23、28为首次从茄科植物中分离得到,化合物2、19、24、27首次从曼陀罗属植物中分离得到。化合物9、20具有较强的抗炎活性。AIM To study the chemical constituents from the leaves of Datura metel L.and their anti-inflammatory activities.METHODS The 70% ethanol extract of the leaves of D.metel was isolated and purified by macroporous resin,silica gel,ODS and HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.Their anti-inflammatory activities in vitro were evaluated by RAW264.7 model.RESULT Twenty eight compounds were isolated and identified as 7,27-dihydroxy-1-oxowitha-2,5,24-trienolide(1),acnistoferin(2),baimantuoluoline K(3),daturafoliside G(4),daturafoliside H(5),daturafoliside I(6),daturafoliside R(7),daturafoliside S(8),daturametelin A(9),daturametelin I(10),daturametelin J(11),daturataturin A(12),baim antuoluoside H(13),(22R)-27-hydroxy-7α-methoxy-1-oxowitha-3,5,24-trienolide(14),corilagin(15),desmethylagrimonolide 6-O-β-D-glucopyranoside(16),agrimonolide-6-O-β-D-glucopyranoside(17),syringaresinol(18),syringaresinol-4-O-β-D-glucopyranoside(19),1,6-di-O-coumaroyl-glucopyranoside(20),scoparone(21),scopoletin(22),rosamultin(23),(-)-loliolide(24),(+)-isololiolide(25),dibutyl phthalate(26),p-hydroxybenzaldehyde(27),ethyl gallate(28).Compounds 9,20 effectively inhibited the production of NO by LPS-induced cells with the IC_(50) value of 25.14,16.26 μmol/L,respectively.CONCLUSION Compounds 15-17,20,23,28 are first isolated from family Solanaceae,compounds 2,19,24,27 are isolated from genus Datura for the first time.Compounds 9 and 20 show strong anti-inflammatory activities.

关 键 词：洋金花 叶 化学成分 分离鉴定 抗炎活性 

分 类 号：R284.1[医药卫生—中药学]