茺蔚子中苷类成分及其肝细胞保护作用研究  

Glycosides from Leonuri Fructus and their hepatoprotective effects

作　　者：范云秋 彭芳 何育霖[1,2] 彭成 戴鸥[1,2] 周勤梅[1,2,3] 熊亮 FAN Yun-qiu;PENG Fang;HE Yu-lin;PENG Cheng;DAI Ou;ZHOU Qin-mei;XIONG Liang(State Key Laboratory for Southwestern Chinese Medicine Resources,School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Institute of Innovative Medicine Ingredients of Southwest Specialty Medicinal Materials,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Institute of Traditional Chinese Medicine Innovation,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)

机构地区：[1]成都中医药大学药学院,省部共建西南特色中药资源国家重点实验室,四川成都611137 [2]成都中医药大学西南特色药材创新药物成分研究所,四川成都611137 [3]成都中医药大学中医药创新研究院,四川成都611137

出　　处：《中成药》2022年第6期1840-1844,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金优秀青年基金(82022072);国家自然科学基金面上项目(81872991);国家自然科学基金“联合基金重点支持项目”(U19A2010);四川省应用基础研究项目(2019YJ0334);成都中医药大学“杏林学者”学科人才提升计划(YXRC2018005)。

摘　　要：目的 研究茺蔚子Leonuri Fructus化学成分及其肝细胞保护作用。方法 对茺蔚子正丁醇提取物采用大孔吸附树脂、HW-40F凝胶以及半制备液相色谱进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用对乙酰氨基酚(APAP)所致HL-7702细胞损伤模型评价其肝细胞保护作用。结果 从中分离得到7个苷类,分别鉴定为β-苯乙醇葡萄糖苷(1)、darendoside B(2)、肉苁蓉苷E(3)、肉苁蓉苷G(4)、熊果苷(5)、2-O-β-葡萄糖基-5-甲氧基-苯甲酸甲酯(6)、对羟基苯甲酰基熊果苷(7)。其中化合物4、5具有肝细胞保护作用,其EC_(50)值分别为(35.71±6.26)、(6.66±0.79)μmol/L。结论 化合物2、4~7均为首次从该植物中分离得到,化合物4、5均具有显著的肝细胞保护作用。AIM To study the chemical constituents from Leonuri Fructus and their hepatocellular protective effect.METHODS The n-butanol extract from Leonuri Fructus was isolated and purified by macroporous adsorption resin and HW-40 F column chromatography and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.Their protective effects activities on hepatocytes were evaluated by acetaminophen(APAP)-induced HL-7702 cell injury model.RESULTS Seven compounds were isolated and identified as β-phenylethanol glucoside(1),darendoside B(2),cistanoside E(3),cistanoside G(4),arbutin(5),2-O-β-glucopyranosyl-5-methoxy-benzoic acid methyl ester(6),lanceoloside A(7).Compounds 4 and 5 exhibited protective effects with EC_(50) values of(35.71±6.26) and(6.66±0.79) μmol/L,respectively.CONCLUSION Compounds 2 and 4-7 are isolated from this plant for the first time.Compounds 4 and 5 show significant hepatoprotective effects.

关 键 词：茺蔚子 苷类 分离鉴定 肝细胞保护 

分 类 号：R284.1[医药卫生—中药学]