股骨头坏死发病机制中非编码RNA的靶标性  

Targeting of non-coding RNAs in the pathogenesis of the osteonecrosis of the femoral head

作　　者：潘钟杰 秦志鸿 郑铁军 丁晓飞[1] 廖世杰[1] Pan Zhongjie;Qin Zhihong;Zheng Tiejun;Ding Xiaofei;Liao Shijie(Departments of Orthopedics and Hand Surgery,The First Affiliated Hospital of Guangxi Medical University,Nanning 530022,Guangxi Zhuang Autonomous Region,China;Collaborative Innovation Center of Regenerative Medicine and Medical Biological Resources Development and Application,Guangxi Medical University,Nanning 530022,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]广西医科大学第一附属医院创伤骨科手外科,广西壮族自治区南宁市530022 [2]广西医科大学再生医学与医用生物资源开发应用协同创新中心,广西壮族自治区南宁市530022

出　　处：《中国组织工程研究》2023年第9期1441-1447,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(82060396),项目负责人:丁晓飞;国家自然科学基金项目(82160809),项目负责人:廖世杰;广西自然科学基金面上项目(2017GXNSFAA198305),项目负责人:丁晓飞;广西自然科学基金青年基金项目(2018GXNSFBA281090),项目负责人:廖世杰。

摘　　要：背景:目前非编码RNA已被证实在多种骨科疾病中发挥重要作用,因此,非编码RNA可能为了解该疾病的病因、发病机制、治疗方法带来新的思路。目的:探索早期诊断、治疗股骨头坏死的方法和思路,综述非编码RNA在股骨头坏死中的研究进展,并提出目前所存在的问题及解决方法。方法:以“股骨头坏死,非编码RNA,miRNA,lncRNA,circRNA”为检索词检索中国知网、万方、维普等数据库,以“osteonecrosis of the femoral head,non-coding RNAs,miRNA,lncRNA,circRNA”为检索词检索PubMed、Medline等数据库,检索时限均为2000年1月至2021年11月。最终纳入67篇文献。结果与结论:(1)非编码RNA的种类非常丰富,大约占所有转录产物的98%,同时占所有RNA的90%左右,根据其功能可分为管家非编码RNA(如tRNA、rRNA、snoRNA)以及调节非编码RNA(如miRNA、lncRNA、circRNA、siRNA);(2)目前miRNAs与激素型股骨头坏死的研究逐渐成为热点,而外泌体lncRNAs、circRNAs与股骨头坏死的相关研究尚未见到报道;(3)多数非编码RNA在发生激素型股骨头坏死时表达下调,大部分非编码RNA通过负向调控其相关靶基因,以促进骨髓间充质干细胞增殖、成骨分化,促进成骨标记物的表达,也可以促进血管内皮细胞生成,从而抑制股骨头缺血性坏死的发生及进展;(4)外泌体携带的非编码RNA也可以在股骨头坏死中发生作用,但目前文献量较少,非编码RNA是否通过外泌体携带所产生的作用目前暂不清楚,在未来的研究中可逐渐完善;(5)利用非编码RNA类似物和抑制剂可以有效抑制非创伤性股骨头坏死的发生发展,可以为非创伤性股骨头坏死的早期诊断和治疗提供新的策略。BACKGROUND:At present,non-coding RNAs have been proven to play an important role in a va riety of orthopedic disease.Therefo re,non-coding RNAs may bring new ideas for understanding the etiology,pathogenesis and treatment of this disease.OBJECTIVE:To explore the methods and ideas of early diagnosis and treatment of the femoral head necrosis,review the research progress on non-coding RNAs in the femoral head necrosis,and put forward the existing pro blems and solutions.METHODS:The keywords of"osteonecrosis of the femoral head,non-coding RNAs,miRNA,IncRNA,circRNA"were used to search articles published between January 2000 and November 2021 on CNKI,Wanfang,VIP,PubMed,and Medline databases.Finally,67 articles were included.RESULTS AND CONCLUSION:(1)The types of non-coding RNAs are very rich,accounting for about 98%of all transcription products and 90%of the functions of all RNAs.According to their functions,they can be divided into housekeeping non-coding RNAs,such as tRNA,rRNA,and snoRNA and regulato ry non-coding RNAs,such as miRNA,IncRNA,circRNA,and siRNA.(2)At present,the research on miRNAs and steroid-induced osteonecrosis of the femoral head is the most popular focus,while the related studies on exosomal IncRNAs and circRNAs and necrosis of the femoral head have not been reported.(3)The expression of most non-coding RNAs is down-regulated in the occurrence of ste roid-induced osteonecrosis of the femoral head,and most non-coding RNAs promote the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells and the generation of osteogenic markers by negatively regulating their related to rget genes,can also promote the formation of vascular endothelial cells and inhibit the occurrence and progression of avascular nec rosis of femoral head.(4)Non-coding RNAs carried by exosomes can also play a role in the femoral head necrosis,but there is little literature at present,and the diffe rence between non-coding RNAs carried by exosomes is not clear at present,which can be gradually improved in fu

关 键 词：外泌体 股骨头坏死 非编码RNA MIRNA lncRNA circRNA 骨坏死 股骨头 糖皮质激素 综述 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R68